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BV711 Rat Anti-Mouse CD70
Product Details
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BD OptiBuild™
CD27 ligand; CD27l, CD27LG; Tnfsf7; Ki-24 Antigen
Mouse (Tested in Development)
Rat F344, also known as Fischer, CDF IgG2b, κ
BALB/c mouse B lymphoma A20.2J
Flow cytometry (Qualified)
0.2 mg/ml
AB_2740411
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Cy is a trademark of Amersham Biosciences Limited.
  11. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
740741 Rev. 1
Antibody Details
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FR70

The FR70 monoclonal antibody specifically binds to mouse CD70, a 30-33-kDa type-II transmembrane glycoprotein belonging to the TNF/NGF superfamily. CD70 is the ligand for CD27 and is expressed primarily on activated B cells. CD70 expression can be induced by activation of splenocytes with lipopolysaccharide and stimulatory antibodies specific for IgM, CD40 or both. mRNA coding for CD70 is transiently expressed after Concanavalin A stimulation of splenocytes or thymocytes. CD70 has been observed to be expressed on dendritic cells of Leishmania major-infected mice. The CD27-CD70 interaction delivers costimulatory signals to T cells and NK cells. It has been reported that FR70 antibody blocks binding of mCD27-Ig fusion protein to mouse Cd70-transfected cells and inhibits some in vitro T-cell- and NK-cell-dependent responses.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm.  BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).  Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

740741 Rev. 1
Format Details
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BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV711
Violet 405 nm
407 nm
713 nm
740741 Rev.1
Citations & References
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View product citations for antibody "740741" on CiteAb

Development References (5)

  1. Akiba H, Miyahira Y, Atsuta M. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med. 2000; 191(2):375-382. (Biology). View Reference
  2. Hartwig UF, Karlsson L, Peterson PA, Webb SR. CD40 and IL-4 regulate murine CD27L expression. J Immunol. 1997; 159(12):6000-6008. (Biology). View Reference
  3. Oshima H, Nakano H, Nohara C. Characterization of murine CD70 by molecular cloning and mAb. Int Immunol. 1998; 10(4):517-526. (Immunogen). View Reference
  4. Takeda K, Oshima H, Hayakawa Y, et al. CD27-mediated activation of murine NK cells. J Immunol. 2000; 164(4):1741-1745. (Biology). View Reference
  5. Tesselaar K, Gravestein LA, van Schijndel GM, Borst J, van Lier RA. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J Immunol. 1997; 159(10):4959-4965. (Biology). View Reference
View All (5) View Less
740741 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.