BD OptiBuild™ BV711 Mouse Anti-Human CD163
Clone MAC2-158 (also known as Mac 2-158; Mac-2–158)
(RUO)
Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
Companion Products
The MAC2-158 monoclonal antibody (also known as Clone MAC 2-158) specifically binds to human CD163. CD163 is also known as Scavenger receptor cysteine-rich type 1 protein M130 (M130), SCARI1 (SR-I1), or Hemoglobin scavenger receptor (HbSR). CD163 is an ~130 kDa type I transmembrane glycoprotein comprised of an extracellular domain with nine cysteine-rich (SRCR) scavenger receptor class B domains followed by a transmembrane region and a short cytoplasmic tail. CD163 is expressed on most peripheral blood monocytes, tissue macrophages, and a subset of dendritic cells. CD163 serves as a high affinity receptor for hemoglobin and haptoglobin and mediates endocytosis of hemoglobin and haptoglobin complexes by macrophages. This scavenging function may protect tissues from hemoglobin-mediated oxidative damage and contribute to the uptake and recycling of iron. CD163 can also reportedly bind to (TNF-a)-like weak inducer of the apoptosis (TWEAK) protein and some pathogenic bacteria. A cleaved, soluble form of CD163 can reportedly play an anti-inflammatory role and serve as a marker for macrophage activation in inflammatory responses. High-affinity binding of the MAC2-158 antibody to CD163 is reportedly unaffected by extracellular calcium levels. This clone can be used to measure CD163 expression in freshly drawn whole blood samples stabilized with commonly used anticoagulants, eg, EDTA, citrate or heparin.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Development References (8)
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Bover LC, Cardó-Vila M, Kuniyasu A, et al. A previously unrecognized protein-protein interaction between TWEAK and CD163: potential biological implications.. J Immunol. 2007; 178(12):8183-94. (Biology). View Reference
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Etzerodt A, Moestrup SK. CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxid Redox Signal. 2013; 18(17):2352-2363. (Biology). View Reference
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Fabriek BO, van Bruggen R, Deng DM, et al. The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria.. Blood. 2009; 113(4):887-92. (Biology). View Reference
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Högger P, Sorg C. Soluble CD163 inhibits phorbol ester-induced lymphocyte proliferation.. Biochem Biophys Res Commun. 2001; 288(4):841-3. (Biology). View Reference
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Jensen AL, Collins J, Shipman EP, Wira CR, Guyre PM, Pioli PA. A subset of human uterine endometrial macrophages is alternatively activated. Am J Reprod Immunol. 2012; 68(5):374-386. (Clone-specific: Flow cytometry). View Reference
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Maniecki MB, Etzerodt A, Moestrup S, Møller J, Graversen J. Comparative assessment of the recognition of domain-specific CD163 monoclonal antibodies in human monocytes explains wide discrepancy in reported levels of cellular surface CD163 expression. Immunobiology. 2011; 216(8):882-890. (Clone-specific). View Reference
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Morganelli PM, Guyre PM. IFN-gamma plus glucocorticoids stimulate the expression of a newly identified human mononuclear phagocyte-specific antigen.. J Immunol. 1988; 140(7):2296-304. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
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PrabhuDas MR, Baldwin CL, Bollyky PL, et al. A Consensus Definitive Classification of Scavenger Receptors and Their Roles in Health and Disease.. J Immunol. 2017; 198(10):3775-3789. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
