Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The 15C4 monoclonal antibody specifically recognizes human Lectin-like oxidized LDL receptor 1 (LOX-1 or hLOX-1). LOX-1 is an ~31 kDa single pass type II membrane glycoprotein that contains an extracellular C-type lectin-like domain. LOX-1 is encoded by OLR1 (Oxidized low density lipoprotein receptor 1). This gene belongs to a subfamily of the NK cell C-type lectin-like (CLEC) receptor family and is likewise known as C-type lectin domain family 8 member A (CLEC8A). LOX-1 (OLR1) is expressed on macrophages, platelets, endothelial cells, fibroblasts, and smooth muscle cells. These cells can upregulate expression of the scavenger receptor in response to inflammatory and oxidative mediators. LOX-1 (OLR1) functions as a scavenger receptor identified as Scavenger receptor class E member 1 (SCARE1 or SR-E1) which plays a role in phagocytosis, endocytosis, and cytokine production. This receptor binds to multiple ligands including lipoproteins, oxidized LDL, C-reactive protein, and heat shock proteins released by apoptotic cells. It may play a role in vascular inflammation and atherosclerosis.
Development References (4)
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Duluc D, Gannevat J, Joo H, et al. Dendritic cells and vaccine design for sexually-transmitted diseases.. Microb Pathog. 2013; 58:35-44. (Clone-specific: Immunohistochemistry). View Reference
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Joo H, Li D, Dullaers M, et al. C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses.. Immunity. 2014; 41(4):592-604. (Clone-specific: Flow cytometry). View Reference
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Li D, Romain G, Flamar AL, et al. Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells.. J Exp Med. 2012; 209(1):109-21. (Immunogen: Flow cytometry). View Reference
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Skinner JA, Zurawski SM, Sugimoto C, et al. Immunologic characterization of a rhesus macaque H1N1 challenge model for candidate influenza virus vaccine assessment.. Clin Vaccine Immunol. 2014; 21(12):1668-80. (Clone-specific: Functional assay). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.