The 159227 monoclonal antibody specifically recognizes MHC class I polypeptide-related sequence A (MICA) which is also known as MIC-A, MHC class I chain-related protein A, or stress inducible class I homolog. This ~70 kDa single-pass type I transmembrane glycoprotein is encoded by MICA (major histocompatibility complex class I chain-related gene A) which is a highly polymorphic gene. MICA is very homologous to another protein, MICB. Both MICA and MICB are homologous to major histocompatibility complex (MHC) class I molecules although they lack association with β2 microglobulin. MICA and MICB each contain three extracellular Ig domains but have no capacity to bind peptides. MICA is expressed on some gut epithelial cells. Its expression by other epithelial cells and cell types, including fibroblasts and endothelial cells, is induced by stress, eg, stress caused by bacterial and viral infections, autoimmunity, transplantation rejection, or cellular transformation. MICA and MICB are ligands for NKG2D (CD314), an activating receptor expressed by natural killer (NK) cells, γδ T cells, CD8+ αβ T cells, and some CD4+ αβ T cells.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.