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BUV661 Mouse Anti-Human CD2
Product Details
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BD OptiBuild™
LFA-2; Lymphocyte function antigen-2; T11/Leu-5; Rosette receptor
Human (Tested in Development)
Mouse BALB/c IgG2a, κ
CD3-activated Human T Lymphocytes
Flow cytometry (Qualified)
0.2 mg/ml
AB_2874318
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV661 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
750106 Rev. 4
Antibody Details
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L303.1

The L303.1 monoclonal antibody specifically recognizes the human CD2 antigen. CD2 is a 45-50 kDa type I transmembrane glycoprotein that also forms the binding site for sheep erythrocytes. The CD2 antigen is present on approximately 75% of normal peripheral blood lymphocytes and 95% to 99% of thymocytes. It is also found on a subset of monocytes (approximately one-third) that might be precursors to dendritic cells. The CD2 antibody reacts with essentially all T lymphocytes and with a subset of NK lymphocytes. CD2 and CD58 have been shown to be coreceptors. The interaction of CD2 antigen and CD58 antigen facilitates antigen recognition by T lymphocytes.

The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP.  Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).

    

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.

750106 Rev. 4
Format Details
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BUV661
The BD Horizon Brilliant™ Ultraviolet 661 (BUV661) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 660-nm. BUV661, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 660-nm (e.g., 670/25 bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV661
Ultraviolet 355 nm
350 nm
660 nm
750106 Rev.4
Citations & References
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Development References (10)

  1. Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
  2. Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin.. Transplantation. 1981; 31(4):283-9. (Biology). View Reference
  3. Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T cell activation via the physiological ligands for CD2 and the T cell receptor.. J Exp Med. 1988; 168(3):1145-56. (Biology). View Reference
  4. Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells.. J Immunol. 1999; 163(11):5920-8. (Biology). View Reference
  5. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  6. Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.. J Immunol. 1981; 126(6):2117-22. (Biology). View Reference
  7. Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen.. Proc Natl Acad Sci USA. 1990; 87(7):2603-7. (Biology). View Reference
  8. MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
  9. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Clone-specific: Functional assay). View Reference
  10. Moingeon P, Chang HC, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T lymphocyte antigen recognition function.. Nature. 1989; 339(6222):312-4. (Biology). View Reference
View All (10) View Less
750106 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.