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      Biotin Rat Anti-Human Podoplanin (PA Tag)
      Biotin Rat Anti-Human Podoplanin (PA Tag)
      Flow cytometric analysis of Podoplanin (PA Tag) expression on viable NCCIT cells.  Cells from the Human NCCIT (Pluripotent embryonal carcinoma, ATCC® CRL-2073™) cell line were stained with either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; dashed lined histogram) or Biotin Rat Anti-Human Podoplanin (PA Tag) antibody (Cat. No. 572181; solid line histogram), followed by BD Pharmingen™ PE Streptavidin (Cat. No. 554061) at 1 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Podoplanin (PA Tag) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Biotin Rat Anti-Human Podoplanin (PA Tag)
      Flow cytometric analysis of Podoplanin (PA Tag) expression on viable NCCIT cells.  Cells from the Human NCCIT (Pluripotent embryonal carcinoma, ATCC® CRL-2073™) cell line were stained with either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; dashed lined histogram) or Biotin Rat Anti-Human Podoplanin (PA Tag) antibody (Cat. No. 572181; solid line histogram), followed by BD Pharmingen™ PE Streptavidin (Cat. No. 554061) at 1 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Podoplanin (PA Tag) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
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      BD Pharmingen™
      AGGRUS; GP36; GP40; Gp38; PA2.26 antigen; PDPN; T1A
      Human (QC Testing)
      Rat IgG2a, λ
      Human Podoplanin Peptide (aa 38-51)
      Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Not Recommended)
      0.5 mg/ml
      10630
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. For U.S. patents that may apply, see bd.com/patents.
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      572181 Rev. 1
      Antibody Details
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      NZ-1

      The NZ-1 monoclonal antibody specifically binds with high affinity to Podoplanin. This antibody was generated by immunizing mice with a 14-residue peptide segment (aa 38-51) from a platelet aggregation-stimulating (PLAG) domain of human Podoplanin. For this reason, this antibody is useful to detect the Podoplanin molecule as well as target proteins, such as antibodies that are labeled with a dodecapeptide (GVAMPGAEDDVV) named PA Tag. Podoplanin is a ~43 kDa mucin-type 1 transmembrane glycoprotein composed of a serine- and threonine-rich intracellular domain, a single transmembrane domain and a short intracellular domain. This multifunctional glycoprotein is expressed on various cell types, including intestinal and thymic epithelial cells, lymphatic endothelial cells, kidney podocytes, alveolar type I cells, fibroblastic reticular cells, macrophages, Th17 cells and osteocytes. Podoplanin expression is upregulated in inflammation and cancer. The binding of Podoplanin to the C-type lectin domain family 1 member B (Clec1B, also known as CLEC-2) on the surface of platelets results in platelet aggregation/activation, thrombosis, lymphatic vessel development, and cancer invasion and metastasis. The Podoplanin (PA Tag) antibody can be used for detecting tagged target proteins in cells that do not express endogenous Podoplanin. This antibody specifically reacts with the human Podoplanin sequence and does not react with Podoplanin sequences from mouse, rat, hamster or dog. Reportedly, this antibody can be used for Western blot, immunohistochemistry, immunoprecipitation or imaging applications.

      572181 Rev. 1
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      572181 Rev.1
      Citations & References
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      View product citations for antibody "572181" on CiteAb

      Development References (5)

      1. Brown ZP, Takagi J. The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology.. Adv Exp Med Biol. 2018; 1105:59-76. (Clone-specific: Western blot). View Reference
      2. Fujii Y, Kaneko M, Neyazaki M, Nogi T, Kato Y, Takagi J. PA tag: a versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin.. Protein Expr Purif. 2014; 95:240-7. (Clone-specific: Flow cytometry, Western blot). View Reference
      3. Fujii Y, Matsunaga Y, Arimori T, et al. Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state.. J Cell Sci. 2016; 129(7):1512-22. (Clone-specific: Immunoprecipitation). View Reference
      4. Kato Y, Kaneko MK, Kuno A, et al. Inhibition of tumor cell-induced platelet aggregation using a novel anti-podoplanin antibody reacting with its platelet-aggregation-stimulating domain.. Biochem Biophys Res Commun. 2006; 349(4):1301-7. (Immunogen: Flow cytometry, Immunohistochemistry, Western blot). View Reference
      5. Tamura-Sakaguchi R, Aruga R, Hirose M, et al. Moving toward generalizable NZ-1 labeling for 3D structure determination with optimized epitope-tag insertion.. Acta Crystallogr D Struct Biol. 2021; 77(Pt 5):645-662. (Clone-specific: Immunoprecipitation). View Reference
      View All (5) View Less
      572181 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.