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Alexa Fluor® 647 Rat Anti-Mouse SAA3
Alexa Fluor® 647 Rat Anti-Mouse SAA3
Multiparameter flow cytometric analysis of SAA3 expression in mouse peritoneal macrophages. C57BL/6 peritoneal exudate cells were fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 (Cat. No. 565411) and either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 557691; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse SAA3 (Cat. No. 566652; Right Panel) at 1 μg/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of SAA3 (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of SAA3 expression in mouse peritoneal macrophages. C57BL/6 peritoneal exudate cells were fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 (Cat. No. 565411) and either Alexa Fluor® 647 Rat IgG2b, κ Isotype Control (Cat. No. 557691; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse SAA3 (Cat. No. 566652; Right Panel) at 1 μg/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of SAA3 (or Ig Isotype control staining) versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
serum amyloid A-3 protein; Saa3; Saa-3; AV098916; l7R3
Mouse (QC Testing)
Rat IgG2b, κ
Mouse SAA3 (aa 20-120) Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869809
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566652 Rev. 1
Antibody Details
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JOR110A

The JOR110A monoclonal antibody specifically recognizes mouse serum amyloid A-3 (SAA3); it does not cross-react with mouse SAA1, SAA2, or SAA4. SAA3 is an acute-phase serum amyloid A protein isoform that is induced by interleukin-1 and secreted by macrophages during inflammation. Epithelial and endothelial cells and adipocytes also express SAA3. Recent studies suggest that SAA3 is involved in bone homeostasis, protection against intestinal bacterial infections, and suppression of tumor immunity.

566652 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566652 Rev.1
Citations & References
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Development References (5)

  1. Hiratsuka S, Watanabe A, Sakurai Y, et al. The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase.. Nat Cell Biol. 2008; 10(11):1349-55. (Biology). View Reference
  2. Lee JM, Kim EK, Seo H, et al. Serum amyloid A3 exacerbates cancer by enhancing the suppressive capacity of myeloid-derived suppressor cells via TLR2-dependent STAT3 activation.. Eur J Immunol. 2014; 44(6):1672-84. (Biology). View Reference
  3. Meek RL, Eriksen N, Benditt EP. Murine serum amyloid A3 is a high density apolipoprotein and is secreted by macrophages.. Proc Natl Acad Sci USA. 1992; 89(17):7949-52. (Biology). View Reference
  4. Tashiro M, Iwata A, Yamauchi M, et al. The N-terminal region of serum amyloid A3 protein activates NF-κB and up-regulates MUC2 mucin mRNA expression in mouse colonic epithelial cells.. PLoS ONE. 2017; 12(7):e0181796. (Biology). View Reference
  5. Thaler R, Sturmlechner I, Spitzer S, et al. Acute-phase protein serum amyloid A3 is a novel paracrine coupling factor that controls bone homeostasis.. FASEB J. 2015; 29(4):1344-59. (Biology). View Reference
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566652 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.