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Alexa Fluor® 647 Mouse Anti-Human SUSD2
Alexa Fluor® 647 Mouse Anti-Human SUSD2
Flow cytometric analysis of SUSD2 expression on human breast adenocarcinoma cell lines. SK-BR-3 (ATCC HTB-30, left panel) and MCF-7 (ATCC HTB-22, right panel) cells were stained with either Alexa Fluor®647 Mouse IgG1, κ Isotype Control (Cat. No. 565571, dotted lines) or Alexa Fluor®647 Mouse Anti-Human SUSD2 (Cat. No. 566657, solid lines) at 1 µg/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of SUSD2 expression on human breast adenocarcinoma cell lines. SK-BR-3 (ATCC HTB-30, left panel) and MCF-7 (ATCC HTB-22, right panel) cells were stained with either Alexa Fluor®647 Mouse IgG1, κ Isotype Control (Cat. No. 565571, dotted lines) or Alexa Fluor®647 Mouse Anti-Human SUSD2 (Cat. No. 566657, solid lines) at 1 µg/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
sushi domain-containing protein 2; Sushi domain (SCR repeat) containing; BK65A6.2; testicular tissue protein Li 190
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human retinoblastoma Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869814
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566657 Rev. 1
Antibody Details
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W5C5

The W5C5 monoclonal antibody specifically reacts with SUSD2 (sushi domain containing 2), which is expressed on mesenchymal stem cells (MSC) and on some cancers and neoplastic cell lines.  It is not detected on hematopoietic cells of the peripheral blood and bone marrow. SUSD2 is a type I transmembrane protein that may interact with galectin-1 and the potential cytokine C10orf99, through which it may affect cell growth and immune responsiveness. It has a large extracellular region that contains a sushi domain (a conserved sequence that is often associated with proteins of the complement system). SUSD2 is a marker that is useful for identifying and isolating MSC from bone marrow and endometrial tissue, and those MSC may be induced to differentiate into a wide variety of cell types. Varying levels of SUSD2 expression may correlate with either suppression or enhancement of the growth of different types of neoplastic cells, presumably via a variety of direct and indirect mechanisms.

566657 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566657 Rev.1
Citations & References
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Development References (8)

  1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Clone-specific). View Reference
  2. Cheng Y, Wang X, Wang P, et al. SUSD2 is frequently downregulated and functions as a tumor suppressor in RCC and lung cancer.. Tumour Biol. 2016; 37(7):9919-30. (Biology). View Reference
  3. Hultgren EM, Patrick ME, Evans RL, Stoos CT, Egland KA. SUSD2 promotes tumor-associated macrophage recruitment by increasing levels of MCP-1 in breast cancer.. PLoS ONE. 2017; 12(5):e0177089. (Biology). View Reference
  4. Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE. A novel marker of human endometrial mesenchymal stem-like cells.. Cell Transplant. 2012; 21(10):2201-14. (Clone-specific). View Reference
  5. Pan W, Cheng Y, Zhang H, et al. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.. Sci Rep. 2014; 4:6812. (Biology). View Reference
  6. Sivasubramaniyan K, Harichandan A, Schumann S, et al. Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2.. Stem Cells Dev. 2013; 22(13):1944-54. (Immunogen: Cell separation, Flow cytometry, Immunofluorescence). View Reference
  7. Ulrich D, Tan KS, Deane J, et al. Mesenchymal stem/stromal cells in post-menopausal endometrium.. Hum Reprod. 2014; 29(9):1895-905. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
  8. Watson AP, Evans RL, Egland KA. Multiple functions of sushi domain containing 2 (SUSD2) in breast tumorigenesis.. Mol Cancer Res. 2013; 11(1):74-85. (Biology). View Reference
View All (8) View Less
566657 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.