Name: Alexa Fluor® 647 Mouse Anti-Human CD164
Brand: BD Pharmingen™
SKU: 561201

Alexa Fluor® 647 Mouse Anti-Human CD164

Clone N6B6

(RUO)

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Scientific Resources

Flow cytometric analysis of CD164 expression on Ramos cells. Human Ramos cells were stained with either Alexa Fluor® 647 Mouse Anti-Human CD164 antibody (Cat. No. 561201; solid line histogram) or with a Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control (Cat. No. 557715; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed on a BD LSR™ II Flow Cytometry System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: MUC-24; Multi-glycosylated core protein 24; MGC-24; Endolyn

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Immunogen: Human Pre-B Cell Line NALM-1

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_10562407

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO

Size 100 Tests Cat No. 557715

561201 Rev. 1

Antibody Details

N6B6

The N6B6 monoclonal antibody specifically binds to a 80–90 kDa, mucin-like molecule present on CD34+ cells during the early stages of B cell, erythroid cell and myelomonocytic cell development. It is also expressed on epithelial cells, peripheral blood monocytes and weakly on lymphocytes. Mucin-like molecules are a new family of glycoproteins present in tissues of the hematopoietic system. They are highly glycosylated polypeptides, containing predominantly O-linked carbohydrate side chains. Reports suggest that CD164 may play a role in hematopoiesis by facilitating the adhesion of CD34+ cells to bone marrow stroma.

561201 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

561201 Rev.1

Citations & References

Development References (2)

Watt SM, Bühring HJ, Rappold I. CD164, a novel sialomucin on CD34(+) and erythroid subsets, is located on human chromosome 6q21. Blood. 1998; 92(3):849-866. (Immunogen: Flow cytometry). View Reference
Zannettino AC, Bühring HJ, Niutta S, Watt SM, Benton MA, Simmons PJ. The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood. 1998; 92(8):2613-2628. (Biology). View Reference

561201 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.