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Biotin Mouse IgG2b, κ Isotype Control
Product Details
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BD Pharmingen™
Mouse BALB/c IgG2b, κ
Flow cytometry, Isotype control (Routinely Tested)
0.5 mg/ml
AB_397263
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

We recommend Biotin Mouse IgG2b, κ Isotype Control (Cat. No. 555741) for immunofluorescent staining of human whole blood.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559531 Rev. 5
Antibody Details
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MPC-11

The MPC-11 antibody has unknown specificity. The MPC-11 cell line was adapted to continuous culture from the tranplantable Merwin Plasmacytoma-11, which was induced by intraperitoneal implantation of a Millipore diffusion chamber into a BALB/c mouse.

559531 Rev. 5
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
559531 Rev.5
Citations & References
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Development References (2)

  1. Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J Exp Med. 1970; 131(3):515-541. (Clone-specific). View Reference
  2. Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
559531 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.