Description
CD57 (Leu-7): Clone HNK-1 is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with membrane extracts of the HSB-2T-lymphoblastoid cell line. CD8 (Leu-2a): Clone SK1 is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes. The CD57 (Leu-7) antigen is a human lymphocyte antigen, Mr 110 kdaltons (kDa), that is a carbohydrate structure associated with myelin-associated glycoprotein (MAG). The CD8 (Leu-2a) antigen is expressed on the 32-kDa α-subunit of a disulfide-linked bimolecular complex. The CD8 antigen binds to Class I major histocompatibility complex (MHC) molecules on antigen-presenting cells (APC), resulting in increased adhesion between the CD8+ T lymphocytes and the APCs. Binding of the CD8 antigen to Class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex may play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3 antigen/T-cell antigen receptor (TCR) complex.
Preparation And Storage
The Simultest reagent is supplied as a combination of CD57 (Leu-7) FITC and CD8 (Leu-2a) PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | | IgM, κ,IgG1, κ | N/A |
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.