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      Anti-Human CD57 FITC/CD8 PE

      BD Simultest™ Anti-Human CD57 FITC/CD8 PE

      (RUO)
      Product Details
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      BD Simultest™
      Human
      Flow cytometry
      RUO
      Phosphate buffered saline with gelatin and 0.1% sodium azide.


      Description

      CD57 (Leu-7): Clone HNK-1 is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with membrane extracts of the HSB-2T-lymphoblastoid cell line.

      CD8 (Leu-2a): Clone SK1 is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes.

      The CD57 (Leu-7) antigen is a human lymphocyte antigen,  Mr 110 kdaltons (kDa), that is a carbohydrate structure associated with myelin-associated glycoprotein (MAG).

      The CD8 (Leu-2a) antigen is expressed on the 32-kDa α-subunit of a disulfide-linked bimolecular complex. The CD8 antigen binds to Class I major histocompatibility complex (MHC) molecules on antigen-presenting cells (APC), resulting in increased adhesion between the CD8+ T lymphocytes and the APCs. Binding of the CD8 antigen to Class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex may play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3 antigen/T-cell antigen receptor (TCR) complex.

      Preparation And Storage

      The Simultest reagent is supplied as a combination of CD57 (Leu-7) FITC and CD8 (Leu-2a) PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.

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      349510 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      CD57 FITC HNK-1 IgM, κ 916
      CD8 PE SK1 IgG1, κ N/A
      349510 Rev. 1
      Citations & References
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      Development References (21)

      1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981; 127(3):1024-1029. (Biology). View Reference
      2. Anderson P, Blue ML, Morimoto C, Schlossman SF. Cross-linking of T3 (CD3) with T4 (CD4) enhances the proliferation of resting T lymphocytes. J Immunol. 1987; 139:678-682. (Biology).
      3. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
      4. Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference
      5. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
      6. Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
      7. Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
      8. Kubagawa H, Abo T, Balch CM, Cooper MD. Biochemical analysis of antigenic determinants on human natural killer cells by HNK-1 (Leu-7) antibody. Fed Proc. 1983; 42:1219. (Biology).
      9. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
      10. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NKP-15) antigens. J Immunol. 1983; 131:1789. (Biology).
      11. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
      12. Ledbetter JA, Frankel AE, Herzenberg. Human Leu T-cell differentiation antigens: quantitative expression on normal lymphoid cells and cell lines. In: Hammerling G, Hammerling U, Kearney J, ed. Monoclonal Antibodies and T Cell Hybridomas: Perspectives and Technical News. New York: Elsevier/North Holland Biomedical Press; 1981:16-22.
      13. Levacher M, Tallet S, Dazza MC, Dournon E, Rouveix B, Pocidalo JJ. T activation marker evaluation in ARC patients treated with AZT. Comparison with CD4+ lymphocyte count in non-progressors and progressors towards AIDS.. Clin Exp Immunol. 1990; 81(2):177-82. (Biology). View Reference
      14. Lewis DE, Puck JM, Babcock GF, Rich RR. Disproportionate expansion of a minor T cell subset in patients with lymphadenopathy syndrome and acquired immunodeficiency syndrome.. J Infect Dis. 1985; 151(3):555-9. (Biology). View Reference
      15. Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
      16. Prince HE, Kreiss JK, Kasper CK, et al. Distinctive lymphocyte subpopulation abnormalities in patients with congenital coagulation disorders who exhibit lymph node enlargement. Blood. 1985; 66:64. (Biology).
      17. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
      18. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
      19. Schubert J, Lanier LL, Schmidt RE. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:711-714.
      20. Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence.. Clin Immunol Immunopathol. 1986; 38(2):161-77. (Biology). View Reference
      21. Terry LA, DiSanto JP, Small TN, Flomenberg N. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:345-346.
      View All (21) View Less
      349510 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.