CD16 BV605

BD Horizon™ CD16 BV605

Name: CD16 BV605
Brand: BD Horizon™
SKU: 663988

Clone 3G8

(ASR)

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Product Details

Brand: BD Horizon™

Alternative Name: CD16;CD16A;FCGR3A;FcγRIIIA;FcRIIIa;CD16B;FCGR3B;FcγRIIIB;FcRIIIb

Reactivity: Human

Isotype: Mouse BALB/c x DBA/2, also known as CD2F1 or CDF1 IgG1, κ

Immunogen: Human polymorphonuclear leukocytes

Application: Flow cytometry

Concentration: 200 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV N409; V MR5, NK80

Regulatory Status: ASR

Product Notices

Caution: If you choose to combine two or more BD Horizon Brilliant™ Dyes in the same multicolor staining cocktail, fluorescent dye interactions may cause staining artifacts without the use of a buffering solution, such as the BD Horizon™ Brilliant Stain Buffer.

663988 Rev. 1

Antibody Details

3G8

The CD16 antibody, clone 3G8, is derived from the fusion of CD2F1 mouse spleen cells with P3U1 myeloma cells.

The CD16 antibodies recognize a 50 to 65-kilodalton (kDa) antigen present on human NK lymphocytes that is the IgG Fc receptor III.

663988 Rev. 1

Format Details

BV605

The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV605

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 605 nm

663988 Rev.1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.