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Purified Mouse Anti-Gαs
Purified Mouse Anti-Gαs
Western blot analysis of Gαs on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Gαs antibody.
Western blot analysis of Gαs on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Gαs antibody.
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Gαs aa. 11-21
Western blot (Routinely Tested)
45 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612671 Rev. 1
Antibody Details
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Guanine nuceotide binding proteins (G proteins) are composed of three subunits, alpha, beta, and gamma to form a trimer complex.  Together these proteins act as signal transducers for a large number of G-protein linked receptors including neurotransmitters, hormones, and signaling molecules.  Ligand binding to the  G-protein linked receptor triggers the release of GDP from the alpha subunit, allowing GTP to bind, dissociating the beta/gamma complex.  The G alpha subunit (G alpha s) is free to bind to other molecules; it is also a GTPase which hydrolyzes the bound GTP to GDP.  Following hydrolysis, the G alpha molecule re-forms as a trimer complex with G beta/gamma. Thus, G proteins can act as a switch, active when bound to GTP, and inactive when bound to GDP.  Cloning of the G alpha protein revealed that it was alternatively spliced into an alpha1 and alpha2 versions.

612671 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612671 Rev.1
Citations & References
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Development References (1)

  1. Tsukamoto T, Toyama R, Itoh H, Kozasa T, Matsuoka M, Kaziro Y. Structure of the human gene and two rat cDNAs encoding the alpha chain of GTP-binding regulatory protein Go: two different mRNAs are generated by alternative splicing. 1991; 88(8):2974-2978. (Biology). View Reference
612671 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.