Purified Hamster Anti-Mouse IL-1β

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BD Pharmingen™ Purified Hamster Anti-Mouse IL-1β

Name: Purified Hamster Anti-Mouse IL-1β
Brand: BD Pharmingen™
SKU: 550605

Clone B122

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Armenian Hamster IgG, λ1

Immunogen: Mouse IL-1β Protein

Application: ELISA Capture (Routinely Tested), Neutralization (Tested During Development), Immunoprecipitation/Western blot (Reported)

Concentration: 0.5 mg/ml

RRID: AB_393777

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified B122 antibody (Cat. No. 550605) is useful as a capture antibody for a sandwich ELISA that measures mouse IL-1β protein levels. Purified B122 antibody can be paired with a biotinylated anti-mouse IL-1β as a detecting antibody, with recombinant mouse IL-1β (Cat. No. 554577) as the standard. Purified B122 antibody should be titrated 1-4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IL-1β ranging from ~2000 pg/ml to 30 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Note: For assay of mouse IL-1β in serum or plasma samples, the BD OptEIA™ Mouse IL-1β ELISA Set, Cat. No. 559603 is recommended.

IP/WB: The B122 antibody has been reported to be useful for immunoprecipitation and Western blotting studies. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Purified NA/LE Hamster Anti-Mouse IL-1β RUO

Size 0.25 mg Cat No. 557539

550605 Rev. 1

Antibody Details

B122

The B122 antibody recognizes the precursor and mature secreted forms of the mouse IL-1β protein. B122 neutralizes the biological activity of mouse IL-1β, but not the biological activities of mouse IL-1α nor human IL-1β. B122 does not recognize mouse IL-2, IL-4, IL-6, IL-10, TNF or IFN-γ. This antibody also recognizes rat IL-1β. The immunogen used to generate the B122 hybridoma was a purified, mature form of the mouse IL-1β protein.

550605 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

550605 Rev.1

Citations & References

Development References (5)

Fei R, Gott K, Edwards B, Schuyler M. Experimental hypersensitivity pneumonitis: in vitro effects of interleukin-2 and interferon-gamma. J Lab Clin Med. 1995; 126(5):485-494. (Clone-specific: ELISA). View Reference
Hogquist KA, Nett MA, Sheehan KC, Pendleton KD, Schreiber RD, Chaplin DD. Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo. J Immunol. 1991; 146(5):1534-1540. (Clone-specific: ELISA, Immunoprecipitation, Neutralization, Western blot). View Reference
Miller BE, Krasney PA, Gauvin DM, et al. Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme. J Immunol. 1995; 154(3):1331-1381. (Clone-specific: ELISA). View Reference
Troy CM, Stefanis L, Prochiantz A, Greene LA, Shelanski ML. The contrasting roles of ICE family proteases and interleukin-1beta in apoptosis induced by trophic factor withdrawal and by copper/zinc superoxide dismutase down-regulation. Proc Natl Acad Sci U S A. 1996; 93(11):5635-5640. (Clone-specific). View Reference
Wu X, Wittwer AJ, Carr LS, Crippes BA, DeLarco JE, Lefkowith JB. Cytokine-induced neutrophil chemoattractant mediates neutrophil influx in immune complex glomerulonephritis in rat. J Clin Invest. 1994; 94(1):337-344. (Clone-specific). View Reference

View All (5)

550605 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.