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Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human CD2 antibody (Cat. No. 753391) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD OptiBuild™ RY586 Mouse Anti-Human CD2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The L303.1 monoclonal antibody specifically recognizes the human CD2 antigen. CD2 is a 45-50 kDa type I transmembrane glycoprotein that also forms the binding site for sheep erythrocytes. The CD2 antigen is present on approximately 75% of normal peripheral blood lymphocytes and 95% to 99% of thymocytes. It is also found on a subset of monocytes (approximately one-third) that might be precursors to dendritic cells. The CD2 antibody reacts with essentially all T lymphocytes and with a subset of NK lymphocytes. CD2 and CD58 have been shown to be coreceptors. The interaction of CD2 antigen and CD58 antigen facilitates antigen recognition by T lymphocytes.
Development References (10)
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Lanier LL, Phillips JH. A map of the cell surface antigens expressed on resting and activated human natural killer cells. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:157-170.
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Bieber CP, Howard FD, Pennock J, Wong J, Shorthouse R, Stinson EB. Preparation, characterization, and primate testing of monoclonal antithymocyte globulin.. Transplantation. 1981; 31(4):283-9. (Biology). View Reference
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Bierer BE, Peterson A, Gorga JC, Herrmann SH, Burakoff SJ. Synergistic T cell activation via the physiological ligands for CD2 and the T cell receptor.. J Exp Med. 1988; 168(3):1145-56. (Biology). View Reference
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Crawford K, Gabuzda D, Pantazopoulos V, et al. Circulating CD2+ monocytes are dendritic cells.. J Immunol. 1999; 163(11):5920-8. (Biology). View Reference
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Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
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Howard FD, Ledbetter JA, Wong J, Bieber CP, Stinson EB, Herzenberg LA. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.. J Immunol. 1981; 126(6):2117-22. (Biology). View Reference
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Koyasu S, Lawton T, Novick D, et al. Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen.. Proc Natl Acad Sci USA. 1990; 87(7):2603-7. (Biology). View Reference
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MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN. Characterization of human blood dendritic cell subsets.. Blood. 2002; 100(13):4512-20. (Biology). View Reference
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Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Clone-specific: Functional assay). View Reference
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Moingeon P, Chang HC, Wallner BP, Stebbins C, Frey AZ, Reinherz EL. CD2-mediated adhesion facilitates T lymphocyte antigen recognition function.. Nature. 1989; 339(6222):312-4. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.