R718 Mouse Anti-Human FoxP3
Multicolor flow cytometric analysis of FoxP3 expressed in resting human peripheral blood lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with FITC Mouse Anti-Human CD4 (Cat. No. 566320) and BD Horizon™ BV421 Mouse Anti-Human CD25 (Cat. No. 562442) antibodies. The cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) followed by intracellular staining with BD Horizon™ R718 Mouse Anti-Human FoxP3 antibody (Cat No. 566935/566936/566937). The pseudocolor density plots showing the corelated expression of FoxP3 versus CD25 (Left Plot) or CD4 versus FoxP3 (Right Plot) were derived from gated events with the forward and side-light scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
BD Horizon™ R718 Mouse Anti-Human FoxP3
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