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      Purified NA/LE Mouse Anti-Armenian and Syrian Hamster IgG1
      Product Details
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      BD Pharmingen™
      Hamster (QC Testing)
      Mouse BALB/c IgG2b, κ
      Pooled Armenian hamster IgG mAb
      Flow cytometry (Routinely Tested), ELISA (Tested During Development)
      1.0 mg/ml
      AB_395201
      No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      ELISA:  For sandwich ELISA (hamster IgG (group 1)), biotin-conjugated mAb G94-56 (Cat. No. 554007) is optimal for detection with purified anti-hamster IgG mAb HIG-632 (Cat. No. 550637) for capture.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554005 Rev. 13
      Antibody Details
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      G94-56

      Based on ELISA, the G94-56 antibody reacts specifically with Armenian and Syrian hamster IgG1 monoclonal antibodies, but has low reactivity with some other hamster IgG groups.  The G94-56 mAb does not react with hamster IgM.

      554005 Rev. 13
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      554005 Rev.13
      Citations & References
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      View product citations for antibody "554005" on CiteAb
      No Citations Found

      No Citations are available for this Product

      554005 Rev. 13

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.