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Purified Mouse Anti-Rat CD11b
Product Details
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BD Pharmingen™
Itgam; Integrin αM chain; Mac-1 α chain; Integrin beta 2 alpha subunit
Rat (QC Testing)
Mouse BALB/c IgA, κ
Rat neutrophils
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Immunoprecipitation, Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554980 Rev. 11
Antibody Details
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The WT.5 monoclonal antibody specifically binds to CD11b, the α subunit of Mac-1 found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554980 Rev. 11
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554980 Rev.11
Citations & References
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Development References (2)

  1. Tamatani T, Kitamura F, Kuida K, et al. Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera. Eur J Immunol. 1993; 23(9):2181-2188. (Immunogen: Immunoprecipitation, Western blot). View Reference
  2. Watanabe T, Arakawa T, Fukuda T, Higuchi K, Kobayashi K. Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta. Am J Pathol. 1997; 150(3):971-979. (Clone-specific: Blocking). View Reference
554980 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.