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      Purified Mouse Anti-Human CD53
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Purified Mouse Anti-Human CD53
      Flow cytometric analysis of CD53 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD53 (Cat. No. 555506; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      Purified Mouse Anti-Human CD53
      Flow cytometric analysis of CD53 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD53 (Cat. No. 555506; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      Product Details
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      BD Pharmingen™
      tetraspanin-25; TSPAN25; tspan-25; MOX44
      Human (QC Testing)
      Mouse IgG1, κ
      Human Peripheral Blood Mononuclear Cells
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
      0.5 mg/ml
      IV N18
      AB_395896
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555506 Rev. 2
      Antibody Details
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      HI29

      The HI29 monoclonal antibody specifically recognizes CD53. CD53 is a 35-42 kDa type III transmembrane glycoprotein that is expressed on all leucocytes, including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates signal transduction in monocytes and B cells. It has the broadest reactivity of all pan-leukocyte reagents used in immunohistology applications.

      555506 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      555506 Rev.2
      Citations & References
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      Development References (3)

      1. Engel P, Wagner N, Tedder TF. CD86 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:703-705.
      2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      3. Olweus J, Lund-Johansen F, Horejsi V. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993; 151(2):707-716. (Biology). View Reference
      555506 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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