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Purified Mouse Anti-Human β2-Microglobulin
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgM, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555550 Rev. 8
Antibody Details
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β2-microglobulin is the 12 kDa β chain which noncovalently associates with the 44 kDa α chain to form the HLA Class I antigen complex. This antibody reacts with free or complexed β2-microglobulin. TÜ99 does not react with β2-microglobulin of other species. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using TUF pretreatment.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555550 Rev. 8
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555550 Rev.8
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Desoye G, Dohr GA, Motter W, et al. Lack of HLA class I and class II antigens on human preimplantation embryos. J Immunol. 1988; 140(12):4157-4159. (Biology). View Reference
  3. Dohr GA, Motter W, Leitinger S, et al. Lack of expression of HLA [corrected] class I and class II molecules on the human oocyte. J Immunol. 1987; 138(11):3766-3770. (Biology). View Reference
555550 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.