Skip to main content Skip to navigation
PE Rat Anti-Mouse P2X7
PE Rat Anti-Mouse P2X7

Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells.

       Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 (Cat. No. 565411) and APC Rat Anti-Mouse CD117 (Cat. No. 553356) antibodies, and either PE Rat IgG2b, κ Isotype Control (Cat. No. 556925; dashed line histogram) or PE Rat Anti-Mouse P2X7 antibody (Cat. No. 565345; solid line histogram). The fluorescence histogram showing P2X7 expression (or Ig Isotype control staining) was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells.

       Right Panel: Mouse splenocytes were stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 (Cat. No. 562922; Left Plots) and FITC Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 555274/561798; Right Plots) antibodies, and either PE Rat IgG2b, κ Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes.

       Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells.

       Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon™ BV421 Rat Anti-Mouse F4/80 (Cat. No. 565411) and APC Rat Anti-Mouse CD117 (Cat. No. 553356) antibodies, and either PE Rat IgG2b, κ Isotype Control (Cat. No. 556925; dashed line histogram) or PE Rat Anti-Mouse P2X7 antibody (Cat. No. 565345; solid line histogram). The fluorescence histogram showing P2X7 expression (or Ig Isotype control staining) was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells.

       Right Panel: Mouse splenocytes were stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 (Cat. No. 562922; Left Plots) and FITC Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 555274/561798; Right Plots) antibodies, and either PE Rat IgG2b, κ Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes.

       Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
P2X7; P2X7 purinoceptor; P2RX7; P2X7R; P2Z receptor; ATP receptor
Mouse (QC Testing)
Rat IgG2b, κ
Mouse Mast Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739198
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565345 Rev. 1
Antibody Details
Down Arrow Up Arrow
1F11

The 1F11monoclonal antibody specifically binds to the P2X7 receptor. P2X7 is also known as the P2X7 Receptor (P2X7R), Purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), P2X7 purinoceptor, P2Z receptor, or ATP receptor. P2X7 is differentially expressed on a wide variety of cell types including T cells, B cells, macrophages, dendritic cells, mast cells, neurons, microglia, and astrocytes. Functional P2X receptors exist as trimers. Each P2X7 monomer contains intracellular N and C termini, two transmembrane domains, and a large extra cellular loop. When present at relatively high levels, extracellular adenosine 5'-triphosphate (ATP) binds to the P2X7 receptor and opens a channel for extracellular cation (eg, Na+, K+, Ca++) influx into cells. The P2X7 receptor-mediated cation influx can cause membrane depolarization and Ca++ mediated signaling cascades that trigger cellular responses, eg, the production and/or release of proinflammatory mediators (eg, IL-1β or IL-18) by cells of the immune system. P2X7 is also implicated in mediating ATP-induced apoptosis of cells. The 1F11 antibody reportedly blocks ATP-mediated cellular activation through P2X7R.

565345 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565345 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (4)

  1. Bulanova E, Bulfone-Paus S. P2 receptor-mediated signaling in mast cell biology. Purinergic Signal. 2010; 6(1):3-17. (Biology). View Reference
  2. Cloning and functional characterisation of the mouse P2X7 receptor. Cloning and functional characterisation of the mouse P2X7 receptor. FEBS Lett. 1998; 439(1-2):26-30. (Biology). View Reference
  3. Idzko M, Ferrari D, Eltzschig HK. Nucleotide signalling during inflammation. Nature. 2014; 509(7500):310-317. (Biology).
  4. Kurashima Y, Amiya T, Nochi T, et al. Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.. Nat Commun. 2012; 3:1034. (Immunogen: Functional assay, Immunoprecipitation, Inhibition, In vivo exacerbation). View Reference
View All (4) View Less
565345 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.