PE Mouse Anti-Rat CD24

BD Pharmingen™ PE Mouse Anti-Rat CD24

Name: PE Mouse Anti-Rat CD24
Brand: PE Mouse Anti-Rat CD24
SKU: 562104

Clone HIS50 (RUO)

Multicolor flow cytometric analysis of CD24 expression on rat bone marrow cells. Lewis rat bone marrow cells were stained with a FITC Mouse Anti-Rat CD45R antibody (Cat. No. 554880/561876) and either PE Mouse IgM, κ Isotype Control (Cat. No. 555584, Left Panel) or a PE Mouse Anti-Rat CD24 antibody (Cat. No. 562104, Right Panel). Flow cytometric fluorescence dot plots showing CD24 expression (or Ig Isotype Control staining) versus CD45R were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: HSA; Heat-stable antigen; Nectadrin; Cd24a; Signal transducer CD24

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgM, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10898342

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

PE Mouse IgM, κ Isotype Control RUO

Size 100 Tests Cat No. 555584

FITC Mouse Anti-Rat CD45R RUO

Size 50 µg Cat No. 561876

FITC Mouse Anti-Rat CD45R RUO

Size 0.5 mg Cat No. 554880

562104 Rev. 1

Antibody Details

HIS50

The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.

562104 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

562104 Rev.1

Citations & References

View product citations for antibody "562104" on CiteAb

Development References (3)

Akashi T, Shirasawa T, Hirokawa K.. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines.. Virchows Arch. 1994; 425(4):399-406. (Biology). View Reference
Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Immunogen: Immunofluorescence, Immunohistochemistry). View Reference
Pearce EI, Ammerlaan WA, Opstelten D.. Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50.. Hybridoma. 1996 February; 15(1):33-42. (Clone-specific: Western blot). View Reference

562104 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.