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PE Mouse Anti-Human Jagged1 (CD339)
PE Mouse Anti-Human Jagged1 (CD339)

Flow cytometric analysis of Human Jagged 1 expression on transfected cells. CHO cells transfected with the human JAG1 gene were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line histogram) or PE Mouse Anti-Human Jagged1 (Cat. No. 565495, solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of the intact transfected CHO cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

Flow cytometric analysis of Human Jagged 1 expression on transfected cells. CHO cells transfected with the human JAG1 gene were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680, dashed line histogram) or PE Mouse Anti-Human Jagged1 (Cat. No. 565495, solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of the intact transfected CHO cells. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

Product Details
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BD Pharmingen™
Jagged-1, hJ1, CD339, JAG1, JAGL1
Human (QC Testing)
Mouse IgG1, κ
Human JAG1 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739264
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565495 Rev. 1
Antibody Details
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MHJ1-152

The MHJ1-152 monoclonal antibody specifically binds to human Jagged1, a type I transmembrane protein that is one of the ligands for the Notch family of receptors. It is expressed in many fetal and adult tissues including stromal and epithelial cells. JAG1 gene mutations are associated with defects of the heart, liver, kidney and other tissues. Through its interaction with the Notch proteins, Jagged1 affects the development of the hematopoietic system and numerous organs. Jagged1 was assigned the CD339 designation at the 8th International Workshop on Human Leucocyte Differentiation Antigens.

565495 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565495 Rev.1
Citations & References
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Development References (3)

  1. Kume T. Ligand-dependent Notch signaling in vascular formation. Adv Exp Med Biol. 2012; 727:210-222. (Biology). View Reference
  2. Murata A, Yoshino M, Hikosaka M, et al. An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.. PLoS ONE. 2014; 9(9):e108535. (Biology). View Reference
  3. Zola H, Swart B, Nicholson I, et al . CD molecules 2005: human cell differentiation molecules . Blood. 2005; 106(9):3123-3126. (Biology). View Reference
565495 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.