PE Mouse Anti-Human CD49c (Integrin α3)

BD Pharmingen™ PE Mouse Anti-Human CD49c (Integrin α3)

Name: PE Mouse Anti-Human CD49c (Integrin α3)
Brand: BD Pharmingen™
SKU: 568718

Clone P1B5 (also known as P1B5)

(RUO)

Flow cytometric analysis of CD49c (Integrin α3) expression on MG-63 cells. Cells from the human MG-63 (Osteosarcoma, ATCC CRL-1427) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CD49c antibody (Cat. No. 568718; solid line histogram). The fluorescent histogram showing CD49c (Integrin α3) expression (or Ig Isotype control staining) was derived from gated events with the side and forward light-scattering events of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Multiparameter flow cytometric analysis of CD49c (Integrin α3) expression on human peripheral blood leucocytes. Human whole blood was stained with either PE Mouse IgG1, k Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD49c antibody (Cat. No. 568718; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of CD49c (Integrin α3) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: ITGA3; integrin alpha 3; alpha 3 subunit of VLA-3 receptor; VLA3a

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human WI-38 VA13 or HT-1080 Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: VIII 80337

Entrez Gene ID: 3675

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

568718 Rev. 1

Antibody Details

P1B5

The P1B5 monoclonal antibody specifically recognizes Integrin alpha 3 (Integrin α3) which is also known as CD49c (CD49 antigen-like family member C) or VLA-3 subunit alpha (VLA3a or VLA-3α). CD49c (Integrin α3) is a 150 kDa type I transmembrane glycoprotein that is encoded by ITGA3 (Integrin subunit alpha 3) which belongs to the Integrin alpha chain family. CD49c (Integrin α3) non-covalently associates with integrin β1 (CD29) to form heterodimeric Integrin α3β1 (CD49c/CD29 or VLA-3 complex). CD49c is primarily expressed on endothelial and epithelial cells (basal epidermal layers). It is weakly expressed on peripheral blood leucocytes, including T cells, B cells, and monocytes, but is not expressed on platelets. The CD49c/CD29 complex serves as an adhesive receptor for extracellular membrane components including fibronectin, collagen, laminin-1, laminin V, and entactin.

568718 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

568718 Rev.1

Citations & References

View product citations for antibody "568718" on CiteAb

Development References (4)

Halasz P, Fleming FE, Coulson BS. Evaluation of specificity and effects of monoclonal antibodies submitted to the Eighth Human Leucocyte Differentiation Antigen Workshop on rotavirus-cell attachment and entry. Cell Immunol. 2005; 236(1-2):179-187. (Clone-specific: Flow cytometry). View Reference
Melssen MM, Olson W, Wages NA, et al. Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma.. Oncoimmunology. 7(10):e1490855. (Immunogen: Blocking, Immunoprecipitation). View Reference
Wayner EA, Gil SG, Murphy GF, Wilke MS, Carter WG. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.. J Cell Biol. 1993; 121(5):1141-52. (Clone-specific: Blocking, Flow cytometry, Immunoprecipitation). View Reference
Zola H. CD49c. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:124.

View All (4)

568718 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.