PE Mouse Anti-Human CD34

BD Pharmingen™ PE Mouse Anti-Human CD34

Name: PE Mouse Anti-Human CD34
Brand: BD Pharmingen™
SKU: 568295

Clone QBEND/10.rMAb (also known as QB-END-10, QBEND10, QBEND/10, QBEnd10,)

(RUO)

Multicolor flow cytometric analysis of CD34 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells (PBMC) were preincubated in the presence of Human BD Fc Block™ (Cat. No. 564219/564220) and stained with BD Horizon™ BV786 Mouse Anti-Human CD14 (Cat. No. 563698), APC Mouse Anti-Human CD133 (Cat. No. 566597) and either PE Mouse IgG1, κ Isotype Control (Cat No. 349043; Left Plot) or PE Mouse Anti-Human CD34 antibody (Cat No. 568294/568295; Right Plot). The pseudocolor density plot showing the correlated expression of CD34 (or Ig Isotype control staining) versus CD133 was derived from gated events with the forward and side-light scatter characteristics of viable (CD14-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CD34; CD34 antigen

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, λ2

Immunogen: Human Placental Vesicles

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO (GMP)

Size 100 Tests Cat No. 349043

Human BD Fc Block™ RUO

Size 0.25 mg Cat No. 564220

BV786 Mouse Anti-Human CD14 RUO

Size 100 Tests Cat No. 563698

APC Mouse Anti-Human CD133 RUO

Size 25 µg Cat No. 566597

View Details

568295 Rev. 2

Antibody Details

QBEND/10.rMAb

The QBEND/10.rMab recombinant monoclonal antibody specifically recognizes human CD34, a 105-120 kDa single-chain type I transmembrane glycoprotein, also known as CD34 antigen. CD34 is expressed on immature hematopoietic progenitor cells and all hematopoietic colony-forming cells in the bone marrow and blood, including vascular endothelium cells and some tissue fibroblasts. Normal peripheral blood lymphocytes, monocytes, granulocytes, and platelets do not express CD34. CD34 density is highest on early hematopoietic progenitor cells and decreases as cells mature. The antigen is absent on fully differentiated hematopoietic cells. The cytoplasmic region of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting CD34 may play a role in signal transduction. CD34 has also been named as a possible adhesion molecule by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. The QBEND/10.rMAb antibody recognizes an epitope on CD34 distinct from those recognized by clones My10, 563, 581, and 8G12.

568295 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

568295 Rev.2

Citations & References

View product citations for antibody "568295" on CiteAb

Development References (6)

Orfao A, Matarraz S, Pérez-Andrés M, et al. Immunophenotypic dissection of normal hematopoiesis. J Immunol Methods. 2019; 475:112684. (Biology: Flow cytometry). View Reference
Radtke S, Görgens A, Kordelas L, et al. CD133 allows elaborated discrimination and quantification of haematopoietic progenitor subsets in human haematopoietic stem cell transplants.. Br J Haematol. 2015; 169(6):868-78. (Biology: Flow cytometry). View Reference
Ramani P, Bradley NJ, Fletcher CD. QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections.. Histopathology. 1990; 17(3):237-42. (Immunogen: Immunohistochemistry). View Reference
Sankey EA, More L, Dhillon AP. QBEnd/10: a new immunostain for the routine diagnosis of Kaposi's sarcoma.. J Pathol. 1990; 161(3):267-71. (Clone-specific: Immunohistochemistry). View Reference
Sutherland DR, Keating A. The CD34 antigen: structure, biology, and potential clinical applications.. J Hematother. 1992; 1(2):115-29. (Clone-specific: Cell separation, Flow cytometry). View Reference
Sutherland DR, Stewart AK, Keating A. CD34 antigen: molecular features and potential clinical applications.. Stem Cells. 1993; 11 Suppl 3:50-7. (Clone-specific: Cell separation, Flow cytometry). View Reference

View All (6)

568295 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.