PE Mouse Anti-Human CD122

BD Pharmingen™ PE Mouse Anti-Human CD122

Name: PE Mouse Anti-Human CD122
Brand: BD Pharmingen™
SKU: 554522

Clone Mik-β2

(RUO)

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL-2 Receptor β chain; IL2RB; IL-2Rβ; IL15RB; P70-75

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Application: Flow cytometry (Routinely Tested), Blocking (Reported)

Concentration: 0.2 mg/ml

Workshop Number: V C045

Entrez Gene ID: 3560

RRID: AB_395451

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

The antibody from clone Mik-β2 has been reported to inhibit dose-dependently the [125]I-IL-2 binding to YTS cells, which express the β chain alone, with a 50% binding inhibition at 3 mg/ml. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

554522 Rev. 1

Antibody Details

Mik-β2

The Mik-β2 monoclonal antibody specifically recognizes the 75 kD β subunit (p75) of the IL-2 receptor (IL-2Rβ). Cell surface IL-2Rβ molecules are expressed by T cells, B cells, monocytes, myeloid precursors, NK cells, and LGL. Together with the α subunit (p55, CD25) and the common γ subunit (CD132; γc subunit of the IL-2R, IL-4R, and IL-7R), the IL-2Rβ molecule forms a high-affinity, signaling receptor complex for IL-2 which can be expressed by activated T and B lymphocytes. Alternatively, some cell types, such as NK cells and myeloid cell populations, coexpress IL-2Rβ molecules and γc subunits to form intermediate-affinity, signalling receptor complexes for IL-2.

554522 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

554522 Rev.1

Citations & References

View product citations for antibody "554522" on CiteAb

Development References (2)

Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Biology). View Reference

554522 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.