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Multicolor flow cytometric analysis of CD301b (Mgl2) expression on viable Mouse bone marrow-derived dendritic cells. C57BL/6 Mouse bone marrow-derived dendritic cells (DCs) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with APC Hamster Anti-Mouse CD11c antibody (Cat. No. 550261) and with either BD Horizon™ PE-CF594 Rat IgG2a, λ Isotype Control (Cat. No. 570171; Left Plot) or BD Horizon™ PE-CF594 Rat Anti-Mouse CD301b (Mgl2) antibody (Cat. No. 569537/569538; Right Plot) at 0.5 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD301b (Mgl2) [or Ig Isotype control staining] versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) bone marrow-derived DCs. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ PE-CF594 Rat Anti-Mouse CD301b (Mgl2)
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Companion Products
The URA-1 monoclonal antibody specifically recognizes the extracellular domain of the mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2) and is encoded by Mgl2 (Macrophage galactose N-acetyl-galactosamine specific lectin 2). Mouse D301b (MGL2) is a ~42 kDa type II transmembrane glycoproteins that is comprised of an extracellular region with a carbohydrate recognition domain (CRD) followed by a transmembrane region and a cytoplasmic tail. CD301b is expressed on conventional dendritic cells (cDCs) and immature DCs. CD301b recognizes molecules having galactose and N-actetylgalactosamine (GalNAc) residues. CD301b is involved in the recognition and endocytosis of glycoproteins and play roles in tissue remodeling, clearance of apoptotic cells, and defense against tumor cells. The ER-MP23 monoclonal antibody specifically recognizes both CD301a and CD301b and can reportedly inhibit their binding of carbohydrate ligands.
Development References (3)
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Denda-Nagai K, Aida S, Saba K, et al. Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by dendritic cells.. J Biol Chem. 2010; 285(25):19193-204. (Immunogen: Blocking, ELISA, Flow cytometry, Immunohistochemistry). View Reference
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Sato K, Imai Y, Higashi N, et al. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a.. Blood. 2005; 106(1):207-15. (Clone-specific: Immunohistochemistry). View Reference
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Tsuiji M, Fujimori M, Ohashi Y, et al. Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1.. J Biol Chem. 2002; 277(32):28892-901. (Biology). View Reference
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