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PE-CF594 Mouse Anti-Human Galectin-3
PE-CF594 Mouse Anti-Human Galectin-3

Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ Isotype Control (Cat. No. 562292; dashed line histogram) or BD Horizon PE-CF594 Mouse Anti-Human Galectin-3 antibody (Cat. No. 565682; solid line histogram) at 1.0 ug/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data on this Technical Data Sheet is not lot specific.

Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either BD Horizon™ PE-CF594 Mouse IgG1, κ Isotype Control (Cat. No. 562292; dashed line histogram) or BD Horizon PE-CF594 Mouse Anti-Human Galectin-3 antibody (Cat. No. 565682; solid line histogram) at 1.0 ug/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data on this Technical Data Sheet is not lot specific.

Product Details
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BD Horizon™
LGALS3; Gal-3; GAL3; GALIG; GALBP; CBP35; MAC-2; IgE-binding protein; L31
Human (QC Testing), Mouse (Reported)
Mouse BALB/c IgG1, κ
Recombinant Human Galectin-3
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869700
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ PE-CF594 under optimum conditions, and unconjugated antibody and free PE-CF594 were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
  5. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
  6. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. CF™ is a trademark of Biotium, Inc.
  10. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  11. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  12. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565682 Rev. 1
Antibody Details
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B2C10

The B2C10 monoclonal antibody specifically recognizes Galectin-3 (GAL3/Gal-3) which is also known as, IgE-binding protein (εBP/IgEBP), Carbohydrate-binding protein 35 (CBP35), Galactoside-binding protein (GALBP), or  MAC-2. Galectin-3 is a 30 kDa protein that is encoded by LGALS3 (lectin, galactoside-binding, soluble, 3) and belongs to the galactoside-binding lectins family. It is expressed by a variety of cell types including monocytes/macrophages, dendritic cells, some activated lymphocytes, fibroblasts, epithelial cells, and tumor cells. Galectin-3 has diverse functions and is involved in the induction and regulation of immune responses, inflammation, cell adhesion, chemotaxis, apoptosis, angiogenesis, and fibrosis. Galectin-3 can be differentially expressed in the nucleus, cytoplasm, and surface of cells and can be secreted. Galectin-3 levels can be markedly elevated in the nuclei of proliferating cells suggesting that it may play a role in cell growth regulation. Nuclear galectin-3 has been associated with gene expression and pre-mRNA splicing. Cell surface Galectin-3 reportedly serves as a non-integrin, laminin-binding protein, suggesting its role in cell adhesion to basement membranes. The B2C10 antibody is suitable for immunohistochemical staining of paraffin-embedded tissue sections. The B2C10 antibody reportedly crossreacts with mouse Galectin-3.

This antibody is conjugated to BD Horizon PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red®. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red® yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496 nm and 564 nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red® (eg 610/20-nm filter).

565682 Rev. 1
Format Details
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PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
565682 Rev.1
Citations & References
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Development References (9)

  1. Acosta-Rodríguez EV, Montes CL, Motrán CC, et al. Galectin-3 mediates IL-4-induced survival and differentiation of B cells: functional cross-talk and implications during Trypanosoma cruzi infection.. J Immunol. 2004; 172(1):493-502. (Clone-specific: Flow cytometry). View Reference
  2. Cherayil BJ, Weiner SJ, Pillai S. The Mac-2 antigen is a galactose-specific lectin that binds IgE. J Exp Med. 1989; 170(6):1959-1972. (Biology). View Reference
  3. Cortegano I, del Pozo V, Cárdaba B, et al. Galectin-3 down-regulates IL-5 gene expression on different cell types. J Immunol. 1998; 161(1):385-389. (Biology). View Reference
  4. Haudek KC, Spronk KJ, Voss PG, Patterson RJ, Wang JL, Arnoys EJ. Dynamics of galectin-3 in the nucleus and cytoplasm.. Biochim Biophys Acta. 2010; 1800(2):181-9. (Biology). View Reference
  5. Liu FT, Hsu DK, Zuberi RI, et al. Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.. Biochemistry. 1996; 35(19):6073-9. (Immunogen: Bioassay, Blocking, ELISA, Functional assay, Inhibition, Radioimmunoassay, Western blot). View Reference
  6. Mey A, Leffler H, Hmama Z, Normier G, Revillard JP. The animal lectin galectin-3 interacts with bacterial lipopolysaccharides via two independent sites. J Immunol. 1996; 156(4):1572-1577. (Biology). View Reference
  7. Newlaczyl AU, Yu LG. Galectin-3--a jack-of-all-trades in cancer.. Cancer Lett. 2011; 313(2):123-8. (Biology). View Reference
  8. Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells.. Lab Invest. 2006; 86(7):724-37. (Clone-specific: Flow cytometry). View Reference
  9. Sano H, Hsu DK, Yu L, et al. Human galectin-3 is a novel chemoattractant for monocytes and macrophages.. J Immunol. 2000; 165(4):2156-64. (Clone-specific: Bioassay, Functional assay, Inhibition). View Reference
View All (9) View Less
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.