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FITC Mouse Anti-Human CD371 (Clec12A)
FITC Mouse Anti-Human CD371 (Clec12A)

Flow cytometric analysis of human CD371 (Clec12A) expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells (PBMC) were stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 556652) or FITC Mouse Anti-Human CD371 (Clec12A) (Cat. No. 562569) antibody. The cells were also stained with PE-Cy™7 Mouse Anti-Human HLA-DR (Cat. No. 560651), Alexa Fluor® 700 Mouse Anti-Human CD11c (Cat. No. 561352), PE-Cy™5 Mouse Anti-Human CD123 (Cat. No. 551065), BD Horizon™ PE-CF594 Mouse Anti-Human CD14 (Cat. No. 562334) and a lineage cocktail comprised of BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366), CD14 (Cat. No. 560350), CD19 (Cat. No. 560354) and CD56 (Cat. No. 560361). Histograms showing the expression of CD371 (solid line) or Ig isotype control staining (dotted line) on CD14+ monocytes (Left Panel), Lineage-HLA-DR+CD11c+ myeloid dendritic cells (Middle Panel) and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells (Right Panel) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes (for dendritic cells), respectively. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.

Flow cytometric analysis of human CD371 (Clec12A) expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells (PBMC) were stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 556652) or FITC Mouse Anti-Human CD371 (Clec12A) (Cat. No. 562569) antibody. The cells were also stained with PE-Cy™7 Mouse Anti-Human HLA-DR (Cat. No. 560651), Alexa Fluor® 700 Mouse Anti-Human CD11c (Cat. No. 561352), PE-Cy™5 Mouse Anti-Human CD123 (Cat. No. 551065), BD Horizon™ PE-CF594 Mouse Anti-Human CD14 (Cat. No. 562334) and a lineage cocktail comprised of BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366), CD14 (Cat. No. 560350), CD19 (Cat. No. 560354) and CD56 (Cat. No. 560361). Histograms showing the expression of CD371 (solid line) or Ig isotype control staining (dotted line) on CD14+ monocytes (Left Panel), Lineage-HLA-DR+CD11c+ myeloid dendritic cells (Middle Panel) and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells (Right Panel) were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes (for dendritic cells), respectively. Flow cytometry was performed using a BD™ LSRII Flow Cytometer System.

Product Details
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BD Pharmingen™
CD371; Clec12A; MICL; CLL-1; DCAL-2
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human CLEC12A Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
X 10-73
160364
AB_11154053
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. CF™ is a trademark of Biotium, Inc.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Cy is a trademark of GE Healthcare.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562569 Rev. 3
Antibody Details
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50C1

The 50C1 monoclonal antibody specifically binds to human CD371 which is also known as Clec12A (C-type lectin domain family 12 member A), C-type lectin-like molecule 1 (CLL-1), myeloid inhibitory C-type lectin-like receptor (MICL), or dendritic cell-associated lectin 2 (DCAL-2). It is expressed on a variety of cells including monocytes, macrophages, dendritic cells, and granulocytes and perhaps some NK cells. Clec12A is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. It is a 30 kDa type II transmembrane glycoprotein that has one single C-type lectin-like domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). Clec12A has similarity with the β-glucan receptor (Dectin-1) and LOX-1 with high N-glycosylation. There are at least five isoforms due to alternative transcript splicing. Signaling through Clec12A can induce internalization of Clec12A, dendritic cell maturation and the production of cytokines including IL-12. Clec12A may also serve as a negative regulator of activated leukocytes recruited to sites of inflammation.

562569 Rev. 3
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
562569 Rev.3
Citations & References
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Development References (5)

  1. Chen CH, Floyd H, Olson NE, et al. Dendritic-cell-associated C-type lectin 2 (DCAL-2) alters dendritic-cell maturation and cytokine production. Blood. 2006; 107(4):1459-1467. (Biology). View Reference
  2. Lahoud MH, Proietto AI, Ahmet F, et al. The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses.. J Immunol. 2009; 182(12):7587-94. (Immunogen: Flow cytometry). View Reference
  3. Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). View Reference
  4. Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). View Reference
  5. van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). View Reference
View All (5) View Less
562569 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.