The 10E5 monoclonal antibody specifically binds to CD226 which is also known as, Platelet and T-cell activation antigen 1 (Pta1), T lineage-specific activation antigen 1 (TLiSA1), or DNAX accessory molecule-1 (DNAM-1). CD226 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD226 serves as an adhesion molecule that is primarily expressed on T lymphocytes. Naïve CD8+ T cells express high levels of CD226 whereas it is differentially expressed on CD4+ T cells. In certain model systems, long term-cloned Th1 cells can express high CD226 levels when compared with the downregulated CD226 expression exhibited on cloned Th2 and Th0 cells. CD226 expression is also detectable on subsets of CD11b+ macrophages and NK cells. Adhesive interactions between CD226 and its ligands, CD112 and CD155, can result in cell signaling events that promote innate and adaptive immune responses, including the differentiation and survival of cytotoxic cells.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.