The WT.3 monoclonal antibody specifically recognizes the 95-100 kDa β2 subunit (CD18), which is found on the majority of leukocytes as a heterodimer with any of the three distinct CD11 α integrin subunits (CD11a or αL, CD11b or αM, CD11c or αX) to form, respectively, LFA-1, Mac-1, and gp150, 95. The function-blocking activity of WT.3 antibody has been determined in several in vitro assays measuring the binding of LFA-1 (αLβ2 integrin) to ICAM-1 (CD54). It has also been reported that WT.3 mAb inhibits leukocyte infiltration in an in vivo system.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.