The HLY9.1.25 monoclonal antibody specifically binds to CD229 which is also known as, Signaling lymphocyte activation molecule family member 3 (SLAMF3), Lymphocyte antigen 9 (Ly9), or T-lymphocyte surface antigen Ly-9. CD229 is a 100-120 kDa, single-pass type I membrane glycoprotein that belongs to the SLAM family within the Ig superfamily. CD229 is expressed on thymocytes, T cells, B cells, dendritic cells, and NK cells. Through homophilic binding, CD229 may play a role in adhesive interactions between T and B cells. During T cell activation, CD229 localizes to the immunological synapse.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.