The 172224 monoclonal antibody specifically recognizes CD300 antigen-like family member A (CD300a) which is also known as Myeloid-associated immunoglobulin-like receptor 1 (MAIR-1 or MAIR-I), Mast cell-derived paired immunoglobulin-like receptor 1 (Mcpir1), CMRF35-like molecule 8 (CLM-8 or Clm8), Leukocyte mono-Ig-like receptor 1 (Lmir1), or Inhibitory receptor protein 60 (IRp60). CD300a is a single-pass type I membrane protein that is encoded by the Cd300a gene which belongs to the immunoglobulin gene superfamily. The extracellular region of CD300a contains an IgV-type domain whereas its intracellular region has three ITIMs and a non-canonical ITIM. CD300a is expressed on some B cells, NK cells, macrophages, dendritic cells, neutrophils, eosinophils, and mast cells. CD300a functions as an inhibitory receptor that can downregulate the effector functions of the cells it is expressed on.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).