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      BUV615 Rabbit Anti-Human PRDX2
      BUV615 Rabbit Anti-Human PRDX2
      Flow cytometric analysis of PRDX2 expression in HEK-293T cells.  Cells from the Human HEK293T (Embryonic Kidney, ATCC® CRL-1573™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either BD Horizon™ BUV615 Rabbit IgG Isotype Control (Cat. No. 570737; dashed line histogram) or BD Horizon™ BUV615 Rabbit Anti-Human PRDX2 antibody (Cat. No. 570740; solid line histogram) at 0.125 µg/test. The fluorescence histogram showing PRDX2 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BUV615 Rabbit Anti-Human PRDX2
      Flow cytometric analysis of PRDX2 expression in HEK-293T cells.  Cells from the Human HEK293T (Embryonic Kidney, ATCC® CRL-1573™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either BD Horizon™ BUV615 Rabbit IgG Isotype Control (Cat. No. 570737; dashed line histogram) or BD Horizon™ BUV615 Rabbit Anti-Human PRDX2 antibody (Cat. No. 570740; solid line histogram) at 0.125 µg/test. The fluorescence histogram showing PRDX2 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      NKEF-B; PRDX2; PRP; PRX2; natural killer cell-enhancing factor B
      Human (QC Testing), Mouse,Rat (Reported)
      Rabbit IgG
      Human Peroxiredoxin-2
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      7001
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

         BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

         For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

      Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
      9. For U.S. patents that may apply, see bd.com/patents.
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      570740 Rev. 1
      Antibody Details
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      EPR5154

      The EPR5154 recombinant monoclonal antibody specifically recognizes Peroxiredoxin-2 (PRDX2), which is also known as PRX2, PRP, NKEF-B or Natural killer cell-enhancing factor B. PRDX2 is a ~ 22 kDa cytosolic protein that is encoded by PRDX2, which belongs to the peroxiredoxin family within the AhpC/Prx1 subfamily of antioxidant defense enzymes. Peroxiredoxins are highly active and important cysteine-dependent peroxidases with redox sensor activity that regulate peroxide and peroxynitrite levels within most tissues and cells. In humans, six isoforms exist (PRDX1-6) and are widely distributed in the cell, PRDX1 (nucleus and cytoplasm), PRDX2 and PRDX6 (cytoplasm), PRDX3 (mitochondria), PRDX4 (endoplasmic reticulum) and PRDX5 (peroxisomes, cytoplasm and mitochondria). PRDX2 is ubiquitously and variably expressed in cells and is the third most abundant protein and one of the leading cytoprotective agents against oxidative damage from reactive oxygen species (ROS). This intracellular thiol-specific peroxidase acts as a sensor of hydrogen peroxide-mediated events by regulating intracellular hydrogen peroxide concentrations and plays a critical role in the modulation of cell survival.  Recent reports have also shown that PRDX2 from macrophages can alter the redox status of cell surface receptors and favor the induction of the inflammatory response by the release of TNF, including the activation of NK cells. This protein may have a proliferative effect and play a role in cancer development as a protumor regulator involved in tumorigenesis and tumor progression.

      570740 Rev. 1
      Format Details
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      BUV615
      The BD Horizon Brilliant™ Ultraviolet 615 (BUV615) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. BUV615, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 615-nm (e.g, 610/20 bandpass filter). The acceptor dye can be excited by the Blue (488-nm) and yellow-green (561-nm) lasers resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BUV615
      Ultraviolet 355 nm
      350 nm
      615 nm
      570740 Rev.1
      Citations & References
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      View product citations for antibody "570740" on CiteAb

      Development References (12)

      1. Ahl PJ, Hopkins RA, Xiang WW, et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.. Communications Biology. 2020; 3(1):1-15. (Clone-specific: Intracellular Staining/Flow Cytometry). View Reference
      2. Chen Y, Yang S, Zhou H, Su D. PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo.. Biomed Res Int. 2020; 2020:8359860. (Biology). View Reference
      3. Fazakerley DJ, Chaudhuri R, Yang P, et al. Mitochondrial CoQ deficiency is a common driver of mitochondrial oxidants and insulin resistance.. Elife. 2018; 7:e32111. (Clone-specific: Western blot). View Reference
      4. Gamradt S, Hasselmann H, Taenzer A, et al. Reduced mitochondrial respiration in T cells of patients with major depressive disorder.. iScience. 2021; 24(11):103312. (Clone-specific: Intracellular Staining/Flow Cytometry). View Reference
      5. Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N. Oxidative Stress and Inflammation: What Polyphenols Can Do for Us?. Oxid Med Cell Longev. 2016; 2016:7432797. (Biology). View Reference
      6. Ishii T, Warabi E, Yanagawa T. Novel roles of peroxiredoxins in inflammation, cancer and innate immunity.. J Clin Biochem Nutr. 2012; 50(2):91-105. (Biology). View Reference
      7. Kang SW, Chae HZ, Seo MS, Kim K, Baines IC, Rhee SG. Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha.. J Biol Chem. 1998; 273(11):6297-302. (Biology). View Reference
      8. Knoops B, Argyropoulou V, Becker S, Ferté L, Kuznetsova O. Multiple Roles of Peroxiredoxins in Inflammation.. Mol Cells. 2016; 39(1):60-4. (Biology). View Reference
      9. Lennicke C, Rahn J, Lichtenfels R, Wessjohann LA, Seliger B. Hydrogen peroxide - production, fate and role in redox signaling of tumor cells.. Cell Commun Signal. 2015; 13:39. (Biology). View Reference
      10. Liu J, Su G, Gao J, Tian Y, Liu X, Zhang Z. Effects of Peroxiredoxin 2 in Neurological Disorders: A Review of its Molecular Mechanisms.. Neurochem Res. 2020; 45(4):720-730. (Biology). View Reference
      11. Stresing V, Baltziskueta E, Rubio N, et al. Peroxiredoxin 2 specifically regulates the oxidative and metabolic stress response of human metastatic breast cancer cells in lungs.. Oncogene. 2013; 32(6):724-35. (Biology). View Reference
      12. Xirouchaki CE, Jia Y, McGrath MJ, et al. Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance.. Sci Adv. 2021; 7(51):eabl4988. (Clone-specific: Western blot). View Reference
      View All (12) View Less
      570740 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.