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APC Rat Anti-Mouse CD18
APC Rat Anti-Mouse CD18

Flow cytometric analysis of CD18 expression on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were stained with either APC Rat anti-Mouse CD18 antibody (Cat. No. 562828, solid line histogram) or APC Rat IgG2a, κ Isotype Control (Cat. No. 553932, dashed line histogram). Flow cytometric histograms were derived from gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.

Flow cytometric analysis of CD18 expression on mouse splenocytes. Splenic leucocytes from C57BL/6 mice were stained with either APC Rat anti-Mouse CD18 antibody (Cat. No. 562828, solid line histogram) or APC Rat IgG2a, κ Isotype Control (Cat. No. 553932, dashed line histogram). Flow cytometric histograms were derived from gated events with the forward and side light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD™ LSR II Flow Cytometry System.

Product Details
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BD Pharmingen™
Itgb2; Integrin beta-2; ITB2; LAD; LCAMB; LFA-1/Mac-1/p150,95 subunit beta
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Cell membrane glycoproteins from mouse T-cell lymphoma BW5147
Flow cytometry (Routinely Tested)
0.2 mg/ml
16414
AB_2737823
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
562828 Rev. 1
Antibody Details
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C71/16

The C71/16 monoclonal antibody specifically binds to mouse CD18 which is also known as the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αxβ2 integrin).  CD18 is a type I transmembrane glycoprotein. Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c).  Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The β2 integrins are important mediators of leukocyte-endothelium interactions.

562828 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
562828 Rev.1
Citations & References
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Development References (3)

  1. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
  2. Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-314. (Biology). View Reference
  3. Trowbridge IS, Omary MB. Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1. J Exp Med. 1981; 154(5):1517-1524. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
562828 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.