Flow cytometric analysis of mouse IgM, κ Isotype Control expression on human peripheral blood lymphocytes. Lymphocytes were left unstained (dashed line histogram) or stained with APC Mouse IgM, κ Isotype Control (Cat. No. 555585; solid line histogram). Fluorescent histograms depicting Ig isotype control expression (or autofluorescence) were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytomety was performed on a BD FACScan™
Flow cytometric analysis of mouse IgM, κ Isotype Control expression on human peripheral blood lymphocytes. Lymphocytes were left unstained (dashed line histogram) or stained with APC Mouse IgM, κ Isotype Control (Cat. No. 555585; solid line histogram). Fluorescent histograms depicting Ig isotype control expression (or autofluorescence) were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytomety was performed on a BD FACScan™
Product Details
BD Pharmingen™
Anti-TNP
Mouse BALB/c IgM, κ
TNP-Keyhole Limpet Hemocyanin
Flow cytometry (Routinely Tested)
20 µl
AB_398606
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555585 Rev. 8
Antibody Details
G155-228
The G155-228 antibody is specific for the hapten trinitrophenol (TNP), a hapten not expressed on human, non-human primate, mouse, or rat primary cells or cell lines. In the absence of specific binding, this immunoglobulin may bind non-specifically to Fc receptors.
555585 Rev. 8
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
555585 Rev.8
Citations & References
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555585 Rev. 8
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.