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Flow cytometric analysis of surface CD179a (VpreB1) expression on Human 293F cells. Human 293F untransfected (dashed line histogram) and transfected (solid line histogram) cells were surface stained with APC Mouse Anti-Human CD179a (VpreB1) antibody (Cat. No. 570346/570416). The fluorescence histograms showing CD179a (VpreB1) expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
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BD Pharmingen™ APC Mouse Anti-Human CD179a (VpreB1)
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The HSL96 monoclonal antibody specifically recognizes CD179a which is also known as VpreB (V pre beta/V pre β). CD179a (VpreB1) is a ~18 kDa, Ig V domain-like protein that is encoded by VPREB1 (pre-B lymphocyte 1). This member of the immunoglobulin (Ig) gene superfamily is primarily expressed in the cytoplasm of normal pro-B and early pre-B cells and at low levels on the surface of early pre-B cells. It is not expressed by normal mature circulating B cells or by other leucocyte populations. CD179a (VpreB1) associates noncovalently with CD179b (λ5) to form CD179a/CD179b (VpreB/ λ5), an Ig light chain-like structure called the surrogate light chain. Surrogate light chains are disulfide-linked to membrane-bound IgM heavy chains in association with signal transducer CD79a/CD79b heterodimers which together form the pre-B cell receptor (preBCR) complex. The preBCR plays a critical role in early B cell proliferation and differentiation. The HSL96 antibody can reportedly be used to stain cytoplasmic and cell surface CD179a (VpreB1).

Development References (6)
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Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
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Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific: Flow cytometry). View Reference
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Matsuo Y, Drexler HG, Okochi A, Sugimoto A, Harashima A, Orita K. Characterization of human B cell-precursor leukemia and mature B-cell leukaemia/lymphoma cell lines: Expression and distribution of human pre-B-cell receptor. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:117-120.
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Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
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Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
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Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
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