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APC Mouse Anti-Human CD179a (VpreB1)
APC Mouse Anti-Human CD179a (VpreB1)
Flow cytometric analysis of surface CD179a (VpreB1) expression on Human 293F cells.  Human 293F untransfected (dashed line histogram) and transfected (solid line histogram) cells were surface stained with APC Mouse Anti-Human CD179a (VpreB1) antibody (Cat. No. 570346/570416). The fluorescence histograms showing CD179a (VpreB1) expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Flow cytometric analysis of surface CD179a (VpreB1) expression on Human 293F cells.  Human 293F untransfected (dashed line histogram) and transfected (solid line histogram) cells were surface stained with APC Mouse Anti-Human CD179a (VpreB1) antibody (Cat. No. 570346/570416). The fluorescence histograms showing CD179a (VpreB1) expression were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Product Details
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BD Pharmingen™
CD179a; IGI; IGVPB; VPREB; VPREB1; VpreB; VpreB1; pre-B lymphocyte 1; v(pre)B protein
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human VpreB/λ5 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl/test
VII 70514
7441
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  4. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. For U.S. patents that may apply, see bd.com/patents.
570416 Rev. 1
Antibody Details
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HSL96

The HSL96 monoclonal antibody specifically recognizes CD179a which is also known as VpreB (V pre beta/V pre β). CD179a (VpreB1) is a ~18 kDa, Ig V domain-like protein that is encoded by VPREB1 (pre-B lymphocyte 1). This member of the immunoglobulin (Ig) gene superfamily is primarily expressed in the cytoplasm of normal pro-B and early pre-B cells and at low levels on the surface of early pre-B cells.  It is not expressed by normal mature circulating B cells or by other leucocyte populations. CD179a (VpreB1) associates noncovalently with CD179b (λ5) to form CD179a/CD179b (VpreB/ λ5), an Ig light chain-like structure called the surrogate light chain. Surrogate light chains are disulfide-linked to membrane-bound IgM heavy chains in association with signal transducer CD79a/CD79b heterodimers which together form the pre-B cell receptor (preBCR) complex. The preBCR plays a critical role in early B cell proliferation and differentiation. The HSL96 antibody can reportedly be used to stain cytoplasmic and cell surface CD179a (VpreB1).

570416 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
570416 Rev.1
Citations & References
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View product citations for antibody "570416" on CiteAb

Development References (6)

  1. Karasuyama H, Lebien TW, Copper MD, Clark EC. CD179 Workshop report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:112-115.
  2. Kiyokawa N, Sekino T, Matsui T, et al. Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.. Mod Pathol. 2004; 17(4):423-9. (Clone-specific: Flow cytometry). View Reference
  3. Matsuo Y, Drexler HG, Okochi A, Sugimoto A, Harashima A, Orita K. Characterization of human B cell-precursor leukemia and mature B-cell leukaemia/lymphoma cell lines: Expression and distribution of human pre-B-cell receptor. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:117-120.
  4. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
  5. Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). View Reference
  6. Zola H, Swart B, Boumsell L, Mason DY. Human Leucocyte Differentiation Antigen nomenclature: update on CD nomenclature. Report of IUIS/WHO Subcommittee.. J Immunol Methods. 2003; 275(1-2):1-8. (Clone-specific: Flow cytometry). View Reference
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570416 Rev. 1

 

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