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      Alexa Fluor™ 647 Rabbit Anti-Human GLUT1
      Alexa Fluor™ 647 Rabbit Anti-Human GLUT1

      Flow cytometric analysis of GLUT1 expression in A549 cells. Cells from the Human A549 (Lung adenocarcinoma, ATCC® CCL-185™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with either Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human GLUT1 antibody (Cat. No. 569293; solid line histogram) at 0.06 µg/test. The fluorescence histogram showing the expression of GLUT1 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

      Alexa Fluor™ 647 Rabbit Anti-Human GLUT1

      Flow cytometric analysis of GLUT1 expression in A549 cells. Cells from the Human A549 (Lung adenocarcinoma, ATCC® CCL-185™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with either Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human GLUT1 antibody (Cat. No. 569293; solid line histogram) at 0.06 µg/test. The fluorescence histogram showing the expression of GLUT1 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

      Product Details
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      BD Pharmingen™
      GLUT; GLUT-1; SLC2A1; glucose transporter type 1, erythrocyte/brain
      Human (QC Testing)
      Rabbit IgG
      Human Glucose Transporter GLUT1
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      6513
      AB_3684946
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      569293 Rev. 2
      Antibody Details
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      EPR3915

      The EPR3915 recombinant monoclonal antibody specifically recognizes Glucose transporter 1 (GLUT1), which is also known as GLUT, GLUT-1, SLC2A1 or Glucose transporter type 1, erythrocyte/brain. GLUT1 is a ~54 kDa integral membrane protein encoded by SLC2A1 (Solute carrier family 2, facilitated glucose transporter member 1), which belongs to the Major Facilitator Superfamily (MFS) of membrane transporters. This transport protein contains 12 hydrophobic transmembrane helices and an intracellular domain with both N- and C-termini within the cytosol. GLUT1 is differentially expressed by a wide variety of normal cells including erythrocytes, blood-brain barrier endothelial cells, astrocytes, adipocytes, cardiomyocytes, lymphocytes, and other leucocytes, as well as by tumor cells and cell lines. GLUT1 serves as a major transporter for glucose, other aldose sugars and vitamin C across the mammalian blood-brain barrier and is a key regulator of glucose metabolism. GLUT1 is essential for CD4+T cell activation and effector T cell (Teff) expansion and survival. Disrupting normal glucose metabolism in immune cells may lead to hyperactive immune responses and pathological disease.

      569293 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      569293 Rev.2
      Citations & References
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      View product citations for antibody "569293" on CiteAb

      Development References (11)

      1. Ahl PJ, Hopkins RA, Xiang WW, et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.. Communications Biology. 2020; 3(1):1-15. (Clone-specific: Flow cytometry). View Reference
      2. Basu S, Hubbard B, Shevach EM. Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells.. J Leukoc Biol. 2015; 97(2):279-83. (Biology). View Reference
      3. Carruthers A, DeZutter J, Ganguly A, Devaskar SU. Will the original glucose transporter isoform please stand up!. Am J Physiol Endocrinol Metab. 2009; 297(4):E836-48. (Biology). View Reference
      4. Chan O, Burke JD, Gao DF, Fish EN. The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis.. J Biol Chem. 2012; 287(35):29406-16. (Biology). View Reference
      5. Fu Y, Maianu L, Melbert BR, Garvey WT. Facilitative glucose transporter gene expression in human lymphocytes, monocytes, and macrophages: a role for GLUT isoforms 1, 3, and 5 in the immune response and foam cell formation.. Blood Cells Mol Dis. 2004; 32(1):182-90. (Biology). View Reference
      6. Gamradt S, Hasselmann H, Taenzer A, et al. Reduced mitochondrial respiration in T cells of patients with major depressive disorder.. iScience. 2021; 24(11):103312. (Clone-specific). View Reference
      7. Kinet S, Swainson L, Lavanya M, et al. Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells.. Retrovirology. 2007; 4:31. (Biology). View Reference
      8. Macintyre AN, Gerriets VA, Nichols AG, et al. The glucose transporter Glut1 is selectively essential for CD4 T cell activation and effector function.. Cell Metab. 2014; 20(1):61-72. (Biology). View Reference
      9. Maciver NJ, Jacobs SR, Wieman HL, Wofford JA, Coloff JL, Rathmell JC. Glucose metabolism in lymphocytes is a regulated process with significant effects on immune cell function and survival.. J Leukoc Biol. 2008; 84(4):949-57. (Biology). View Reference
      10. Manel N, Kim FJ, Kinet S, Taylor N, Sitbon M, Battini JL. The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV.. Cell. 2003; 115(4):449-59. (Biology). View Reference
      11. Takenouchi N, Jones KS, Lisinski I, et al. GLUT1 is not the primary binding receptor but is associated with cell-to-cell transmission of human T-cell leukemia virus type 1.. J Virol. 2007; 81(3):1506-10. (Biology). View Reference
      View All (11) View Less
      569293 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.