PE Mouse IgG2a, κ Isotype Control
Expression of human MIP-1α by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (100 ng/ml final concentration) in the presence of GolgiStop™ (2 µM final concentration; Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD14 monoclonal antibody (FITC-M5E2, Cat. No. 555397), fixed permeabilized, and subsequently stained with either PE-anti-human MIP-1α (Cat. No. 554730, left panel), or PE-mouse IgG2a, κ (Cat. No. 559319; middle panel), by following the Pharmingen staining protocols. The data reflect gating on monocytes, based on forward and scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls (right panel).
BD Pharmingen™ PE Mouse IgG2a, κ Isotype Control
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