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Anti-Human IFN-γ FITC/IL-4 PE
Product Details
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BD FastImmune™
Human
Flow cytometry
RUO (GMP)
Phosphate buffered saline with gelatin and 0.1% sodium azide.


Description

The Anti-Hu–IFN-γ antibody, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

The Anti-Hu–IL-4 antibody, clone 3010.211, is derived from the hybridization of Sp2/0 mouse cells with the spleen cells from BALB/c mice immunized with recombinant human IL-4.

The Anti-Human Interferon-γ (Anti-Hu–IFN-γ) antibody recognizes a 20- to 25-kilodalton (kDa) glycoprotein. Anti-Human Interleukin-4 (Anti-Hu–IL-4) recognizes a 15- to 19-kDa glycoprotein.


The Anti-Hu–IFN-γ antibody, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

The Anti-Hu–IL-4 antibody, clone 3010.211, is derived from the hybridization of Sp2/0 mouse cells with the spleen cells from BALB/c mice immunized with recombinant human IL-4.

The Anti-Human Interferon-γ (Anti-Hu–IFN-γ) antibody recognizes a 20- to 25-kilodalton (kDa) glycoprotein. Anti-Human Interleukin-4 (Anti-Hu–IL-4) recognizes a 15- to 19-kDa glycoprotein.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340456 Rev. 1
Components
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Description Clone Isotype
IFN-γ FITC TgMab-2 IgG1, κ
IL-4 PE 3010.211 IgG1,
340456 Rev. 1
Citations & References
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Development References (15)

  1. Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
  2. Bradding P, Feather IH, Howarth PH, et al. Interleukin 4 is localized to and released by human mast cells. J Exp Med. 1992; 176:1381-1386. (Biology).
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
  6. Elliott MJ, Gamble JR, Park LS, Vadas MA, Lopez AF. Inhibition of human monocyte adhesion by interleukin-4. Blood. 1991; 77:2739-2745. (Biology).
  7. Hardy KJ, Sawada T. Human γ interferon strongly upregulates its own gene expression in peripheral blood lymphocytes. J Exp Med. 1989; 170:1021-1026. (Biology).
  8. Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Biology). View Reference
  9. Johnson HM, Bazer FW, Szente BE, Jarpe MA. How interferons fight disease. Scientific American. 1994; May:68-75. (Biology).
  10. Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). View Reference
  11. Paliard X, Malefijt RDW, Yssel H, et al. Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol. 1988; 141:849-855. (Biology).
  12. Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood. 1991; 77(9):1859-1870. (Biology). View Reference
  13. Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology).
  14. Romagnani S, Del Prete G, Maggi E, et al. Human TH1 and TH2 subsets. Int Arch Allergy Immunol. 1992; 99:242-245. (Biology).
  15. Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology).
View All (15) View Less
340456 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

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