Elevating standardization and collaboration to a new level
The BD FACSLyric is designed to enable assay reproducibility across instruments and time.
Universal Setup ensures reproducible and accurate results day to day with stable instrument performance of <0.4%. (Figure 4)
- Universal Setup, a series of built-in daily performance checks
- Optimal instrument performance is achieved through one-tube daily CS&T 1 as part of universal setup
Figure 4: Within-instrument reproducibility of CS&T 1 target MFI's.
For each fluorescence channel, the median of BD™ CS&T 1 bright beads MFI generated from the BD FACSLyric assay setup reports was compared with assay reference target values. The % difference between target and measured was calculated and is shown.
The BD FACSLyric was designed with collaboration in mind.
The unique assay and portability feature enables the sharing and exchange of data, ideas and user-defined protocols within and between institutions.
- User-defined assays are saved and electronically transferred via USB or email to another FACSLyric system
- The time required to set up additional instruments is reduced
- Assay portability simplifies and standardizes instrument setup within your lab and between labs, making collaboration effective and efficient
Figure 5: User-defined assay across three instruments shows the reproducible and accurate performance of the BD FACSLyric Universal Setup.
Assay portability and sharing in 4 easy steps
Strengthening partnerships and expanding global collaborations through assay portability and sharing.
Highly reproducible results between instruments drive standardization (see Table 1 and Figure 6)
|BD FACSLyric Variance (CVs)
|15 BD FACSLyric systems: Lyse/Wash settings and BD FC beads
Table 1. Between-instrument reproducibility of target MFI values on the BD FACSLyric
Lyse/wash assay settings were imported across 15 instruments to show effects of standardization on beads. the CVs of the fluorescence intensity across all channels varies by less than 15.3%. (Figure 5) Daily QC with one lot of BD CS&T 1 beads was run on fifteen BD FACSLyric cytometers. For each instrument, the PMTV gains were automatically adjusted to meet the target values. BD FC beads 1 acquired on each BD FACSLyric instrument. The MFI of positive populations was measured for all parameters across all instruments. The %CV is shown.
Figure 6: Corresponding overlays of 15 BD FACSLyric systems show between-instrument variability of less than 8% for the red and blue channels