描述
This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining. This kit also provides an alternative protein transport inhibitor, BD GolgiPlug™ containing brefeldin A. Sufficient BD GolgiPlug reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10⁶ cells/ml.
Components Quantity Fixation/Permeabilization solution 125 mL BD Perm/Wash™ buffer (10x) 100 mL BD GolgiPlug protein transport inhibitor containing brefeldin A 1 mL
| 描述 | 数量/尺寸 | 零件号 | EntrezGene ID |
|---|---|---|---|
| Fixation/Permeabilization solution | 125 mL (1 ea) | 51-2090KZ | N/A |
| BD Perm/Wash™ Buffer | 100 mL (1 ea) | 51-2091KZ | N/A |
| Protein Transport Inhibitor (containing Brefeldin A) | 1 Each (1 ea) | 51-2301KZ | N/A |
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
