Analysis of Sox1 in H9 Embryonic Stem (ES) cells (left panel), H9-derived Neural Stem Cells (NSC) (middle panel), and H9-derived Neurons (right panel). H9 human ES cells (WiCell, Madison, WI), H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), and permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050). All cells were then blocked with 10% mouse serum (Sigma M9505) and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (dashed histograms, Cat. No. 554680) or PE Mouse Anti-Human Sox1 monoclonal antibody (solid lines). Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR™ II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population.
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Analysis of Sox1 in H9 Embryonic Stem (ES) cells (left panel), H9-derived Neural Stem Cells (NSC) (middle panel), and H9-derived Neurons (right panel). H9 human ES cells (WiCell, Madison, WI), H9-derived NSC, and H9-derived neurons were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), and permeabilized with BD™ Phosflow Perm buffer III (Cat. No. 558050). All cells were then blocked with 10% mouse serum (Sigma M9505) and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (dashed histograms, Cat. No. 554680) or PE Mouse Anti-Human Sox1 monoclonal antibody (solid lines). Histograms were derived from gated events based on light scattering characteristics of the ES, NSC, and neurons, respectively. Flow cytometry was performed on a BD LSR™ II flow cytometry system. In the right panel, the brighter peak consists of undifferentiated NSC and glial cells that are staining positive for Sox1 and the dimmer peak consists of the neuronal population.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Human Sox1 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_10714631
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
561592 Rev. 1
Antibody Details
N23-844
The N23-844 monoclonal antibody reacts with human Sox1, a member of the SOX [SRY (sex determining region Y)-HMG-box] family of transcription factors. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. It is one of the earliest transcription factors to be expressed in ectodermal cells committed to the neural fate. Sox1 is expressed in both embryonic and somatic neural stem and progenitor cells, and it is down regulated during neuronal differentiation in many neuronal subtypes.
561592 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561592 Rev.1
Citations & References
Development References (4)
-
Kan L, Israsena N, Zhang Z, et al. Sox1 acts through multiple independent pathways to promote neurogenesis. Dev Biol. 2004; 15;26(2):580-594. (Biology). View Reference
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Malas S, Duthie SM, Mohri F, Lovell-Badge R, Episkopou V. Cloning and mapping of the human SOX1: a highly conserved gene expressed in the developing brain. Mamm Genome. 1997; 8(11):866-868. (Biology). View Reference
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Pevny LH, Sockanathan S, Placzek M, Lovell-Badge R. A role for SOX1 in neural determination. Development. 1998; 125(10):1967-1978. (Biology). View Reference
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Wilson M, Koopman P. Matching SOX: partner proteins and co-factors of the SOX family of transcriptional regulators. Curr Opin Genet Dev. 2002; 12(4):441-446. (Biology). View Reference
561592 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
