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      Purified NA/LE Mouse Anti-Human CD25
      Purified NA/LE Mouse Anti-Human CD25
      Flow cytometric analysis of CD25 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified NA/LE Mouse Anti-Human CD25 (Cat. No. 555429; solid line histogram) or Purified NA/LE Mouse IgG1 κ Isotype Control (Cat. No. 554721; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was carried out on a BD FACScan™ system.
      Purified NA/LE Mouse Anti-Human CD25
      Flow cytometric analysis of CD25 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified NA/LE Mouse Anti-Human CD25 (Cat. No. 555429; solid line histogram) or Purified NA/LE Mouse IgG1 κ Isotype Control (Cat. No. 554721; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was carried out on a BD FACScan™ system.
      Product Details
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      BD Pharmingen™
      IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55
      Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
      Mouse BALB/c IgG1, κ
      Phytohemagglutinin stimulated human lymphocytes
      Flow cytometry (Routinely Tested), Functional assay (Tested During Development)
      1.0 mg/ml
      IV A053
      3559
      AB_395823
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555429 Rev. 10
      Antibody Details
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      M-A251

      The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as  low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα). CD25 is expressed on regulatory T cells,  activated lymphocytes (T and B), and monocytes. It associates with the IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses.

      555429 Rev. 10
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      555429 Rev.10
      Citations & References
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      Development References (2)

      1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      555429 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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