Skip to main content Skip to navigation
PerCP-Cy™5.5 Rat Anti-Mouse CD38
PerCP-Cy™5.5 Rat Anti-Mouse CD38
Multicolor flow cytometric analysis of CD38 expression on BALB/c mouse spleen. Splenocytes were stained simultaneously with PE Hamster anti-Mouse CD3 antibody (Cat. No. 553063/553064/561824) and with either PerCP-Cy™5.5 Rat IgG2a, κ Isotype Control (Cat. No. 550765; Left Panel) or PerCP-Cy™5.5 Rat anti-Mouse CD38 (Cat. No. 562770; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD38 (or Ig Isotype Control staining) versus CD3 for gated events with the forward and side light-scatter characteristics of viable spleen cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD38 expression on BALB/c mouse spleen. Splenocytes were stained simultaneously with PE Hamster anti-Mouse CD3 antibody (Cat. No. 553063/553064/561824) and with either PerCP-Cy™5.5 Rat IgG2a, κ Isotype Control (Cat. No. 550765; Left Panel) or PerCP-Cy™5.5 Rat anti-Mouse CD38 (Cat. No. 562770; Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD38 (or Ig Isotype Control staining) versus CD3 for gated events with the forward and side light-scatter characteristics of viable spleen cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; I-19; NIM-R5
Mouse (QC Testing)
Rat IgG2a, κ
Mouse Bone Marrow Pre-B cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
12494
AB_2737782
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  5. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
  6. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Cy is a trademark of GE Healthcare.
  9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562770 Rev. 2
Antibody Details
Down Arrow Up Arrow
90/CD38

The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes. In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells. CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages. Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells. The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation. CD31, both human and mouse, is reported to be a ligand for human CD38.

562770 Rev. 2
Format Details
Down Arrow Up Arrow
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
562770 Rev.2
Citations & References
Down Arrow Up Arrow

Development References (11)

  1. BD Biosciences Pharmingen. Unpublished results. .
  2. Bean AG, Godfrey DI, Ferlin WG, et al. CD38 expression on mouse T cells: CD38 defines functionally distinct subsets of alpha beta TCR+CD4-CD8- thymocytes. Int Immunol. 1995; 7(2):213-221. (Biology). View Reference
  3. Cockayne DA, Muchamuel T, Grimaldi JC, et al. Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses. Blood. 1998; 92(4):1324-1333. (Biology). View Reference
  4. Deaglio S, Morra M, Mallone R, et al. Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member. J Immunol. 1998; 160(1):395-402. (Biology). View Reference
  5. Erickson LD, Vogel LA, Cascalho M, et al. B cell immunopoiesis: visualizing the impact of CD40 engagement on the course of T cell-independent immune responses in an Ig transgenic system. Eur J Immunol. 2000; 30(11):3121-3131. (Clone-specific: Immunofluorescence). View Reference
  6. Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998; 330(3):1129-1135. (Biology). View Reference
  7. Howard M, Grimaldi JC, Bazan JF, et al. Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38. Science. 1993; 262(5136):1056-1059. (Biology). View Reference
  8. Lund F, Solvason N, Grimaldi JC, Parkhouse RM, Howard M. Murine CD38: an immunoregulatory ectoenzyme. Immunol Today. 1995; 16(10):469-473. (Biology). View Reference
  9. Oliver AM, Martin F, Kearney JF. Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997; 158(3):1108-1115. (Immunogen). View Reference
  10. Oliver AM. Personal Communication. .
  11. Randall TD, Lund FE, Howard MC, Weissman IL. Expression of murine CD38 defines a population of long-term reconstituting hematopoietic stem cells. Blood. 1996; 87(10):4057-4067. (Biology). View Reference
View All (11) View Less
562770 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.