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PerCP Mouse Anti-Human CD69
Product Details
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BD™
AIM; CLEC2C; EA1; GP32/28; Leu-23; MLR-3; VEA; BL-AC/P26
Human
Mouse BALB/c IgG1, κ
CD8+ alloantigen-directed CTL cell line
Flow cytometry
50 μg/mL
20 μL
969
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 100 μg in 2.0 mL (50 μg/mL) of PBS. The PE conjugate is supplied as 3.0 μg in 1.0 mL (3.0 μg/mL) of PBS. The PerCP conjugate is supplied as 50 μg in 1.0 mL (50 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. The APC conjugate is supplied as 100 μg in 0.5 mL (200 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340548 Rev. 1
Antibody Details
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L78

The CD69 antibody, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen–directed cytotoxic T-lymphocyte (CTL) cell line.

The CD69 antibody recognizes a very early human activation antigen that is a disulfide-bonded homodimer consisting of Mr 60-kilodalton (kDa) polypeptides with one or two N-linked oligosaccharides.

340548 Rev. 1
Format Details
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PerCP
PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP
Blue 488 nm
481 nm
675 nm
340548 Rev.1
Citations & References
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Development References (10)

  1. Borrego F, Robertson MJ, Ritz J, Peña J, Solana R. CD69 is a stimulatory receptor for natural killer cell and its cytotoxic effect is blocked by CD94 inhibitory receptor.. Immunology. 1999; 97(1):159-65. (Biology). View Reference
  2. Chen JH, Prince H, Buck D, et al. Leu-23: an early activation antigen on human lymphocytes. Fed Proc. 1988; 2:A1214. (Biology).
  3. Graber M, Bockenstedt LK, Weiss A. Signaling via the inositol phospholipid pathway by T cell antigen receptor is limited by receptor number.. J Immunol. 1991; 146(9):2935-43. (Biology). View Reference
  4. Lanier LL, Buck DW, Rhodes L, et al. Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.. J Exp Med. 1988; 167(5):1572-85. (Biology). View Reference
  5. Pisegna S, Zingoni A, Pirozzi G, et al. Src-dependent Syk activation controls CD69-mediated signaling and function on human NK cells.. J Immunol. 2002; 169(1):68-74. (Biology). View Reference
  6. Schwarting R, Biedobitek G, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:428-432.
  7. Testi R, Philips J, Lanier LL. Constitutive expression of a phosphorylated activation (Leu-23) by CD3 bright thymocytes. J Immunol. 1988; 141:2257. (Biology).
  8. Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
  9. Testi R, Phillips JH, Lanier LL. T cell activation via Leu-23 (CD69).. J Immunol. 1989; 143(4):1123-8. (Biology). View Reference
  10. Testi R, Pulcinelli F, Frati L, Gazzaniga PP, Santoni A. CD69 is expressed on platelets and mediates platelet activation and aggregation.. J Exp Med. 1990; 172(3):701-7. (Biology). View Reference
View All (10) View Less
340548 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.