Purified Mouse Anti-Caspase-3/CPP32

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BD Transduction Laboratories™ Purified Mouse Anti-Caspase-3/CPP32

Name: Purified Mouse Anti-Caspase-3/CPP32
Brand: BD Transduction Laboratories™
SKU: 611049

Clone 46/Caspase-3

(RUO)

Western blot analysis of Caspase-3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of Caspase-3.

Immunofluorescence staining of RSV-3T3 cells.

Western blot analysis of Caspase-3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of Caspase-3.

Immunofluorescence staining of RSV-3T3 cells.

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Product Details

Brand: BD Transduction Laboratories™

Alternative Name: CPP32

Reactivity: Mouse (QC Testing)

Isotype: Mouse IgG1

Immunogen: Mouse CPP32 aa. 25-145

Application: Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development)

Target Molecular Weight: 32 kDa

Concentration: 250 µg/ml

RRID: AB_398361

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

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Antibody Details

46/Caspase-3

Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is a member of the family of cysteine proteases which includes interleukin-1beta-converting enzyme (ICE) and C. elegans protein, Ced-3. An apoptotic signal such as granzyme B of cytotoxic T-cells (CTLs) or ICE-like proteases induces the intracellular cleavage of Caspase-3 from the inactive proform (32kDa) to the active form which consists of the p20, p17, and p12 subunits. The active form of Caspase-3 cleaves several other apoptotic proteins including proteins such as DNA fragmentation factor (DFF). Apoptosis can be inhibited by coexpression of Bcl-2 as well as inhibitors of Caspase-3 or other members of the family of cysteine proteases. This antibody recognizes the mouse 32 kDa pro-Caspase-3 and the p17 cleaved form of Caspase 3 in T cell lymphocytes treated with camptothecin.

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Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

611049 Rev.1

Citations & References

Development References (5)

Fernandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J Biol Chem. 1994; 269(49):30761-30764. (Biology). View Reference
Hatai T, Matsuzawa A, Inoshita S, et al. Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation. J Biol Chem. 2000; 275(34):26576-26581. (Clone-specific: Western blot). View Reference
Jia L, Patwari Y, Srinivasula SM, et al. Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis. Oncogene. 2001; 20(35):4817-4826. (Clone-specific: Western blot). View Reference
Li S, Zhao Y, He X, et al. Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis. J Biol Chem. 2002; 277(30):26912-26920. (Clone-specific: Western blot). View Reference
Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell. 1996; 86(1):147-157. (Biology). View Reference

View All (5)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.