Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Bcl-x is related to the Bcl-2 protein and can function independently of Bcl-2 in regulating apoptosis (programmed cell death). The bcl-x transcript is expressed in a number of tissues, with the highest levels in the lymphoid and central nervous systems. Two distinct cDNA species, bcl-xL and bcl-xS, have been observed and appear to arise from alternate 5' splice sites located within the first coding exon of the bcl-x gene. Bcl-xL is composed of 233 amino acids and is similar in size and structure to Bcl-2. The Bcl-xS polypeptide shows a deletion of 63 amino acids which consist of the region of Bcl-xL with the highest degree of amino acid identity to Bcl-2. Like Bcl-2, Bcl-xL inhibits cell death upon growth factor withdrawal when transfected into an IL-3-dependent cell line. However, Bcl-xS inhibits Bcl-2 mediated cell survival.
Development References (5)
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Anderson JS, Teutsch M, Dong Z, Wortis HH. An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis. Proc Natl Acad Sci U S A. 1996; 93(26):10966-10971. (Biology). View Reference
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Devarajan P, De Leon M, Talasazan F, Schoenfeld AR, Davidowitz EJ, Burk RD. The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways. J Biol Chem. 2001; 276(44):40599-40605. (Clone-specific: Western blot). View Reference
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Kishimoto H, Sprent J. Strong TCR ligation without costimulation causes rapid onset of Fas-dependent apoptosis of naive murine CD4+ T cells. J Immunol. 1999; 163(4):1817-1826. (Clone-specific: Flow cytometry). View Reference
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Oetzel C, Jonuleit T, Gotz A, et al. The tyrosine kinase inhibitor CGP 57148 (ST1 571) induces apoptosis in BCR-ABL-positive cells by down-regulating BCL-X. Clin Cancer Res. 2000; 6(5):1958-1968. (Clone-specific: Western blot). View Reference
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Soini Y, Kinnula V, Kaarteenaho-Wiik R, Kurttila E, Linnainmaa K, Paakko P. Apoptosis and expression of apoptosis regulating proteins bcl-2, mcl-1, bcl-X, and bax in malignant mesothelioma. Clin Cancer Res. 1999; 5(11):3508-3515. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
