Description
BD Procount™ progenitor cell enumeration kit is intended for use as an in vitro diagnostic test to identify and enumerate absolute counts and percentages of CD34+ cells in human peripheral blood samples, mobilized peripheral blood, and leukapheresis sample using flow cytometry.
Preparation And Storage
It is important that you carefully follow these instructions. Store the reagent vials upright at 2° to 8°C. Use opened vials within 4 weeks. Expiration dating for closed vials is shown on the label.
Store BD Trucount tubes in their original foil pouch at 2° to 25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch. Once the pouch has been opened, the tubes are stable for 1 month. Do not use beyond the expiration date indicated on the packaging.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Combination of CD34 PE, CD45 PerCP, nucleic acid dye | 25 Tests (1 ea) | 91-0242 | N/A |
| Isotype Controls | 25 Tests (1 ea) | 91-0243 | N/A |
| BD Trucount tubes (25 tubes) | 50 Tests (2 ea) | 91-0786 | N/A |
Development References (10)
-
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
-
Chen CH, Lin W, Shye S, et al. Automated enumeration of CD34+ cells in peripheral blood and bone marrow. J Hematother. 1994; 3-13. (Biology).
-
Clinical and Laboratory Standards Institute. 2005. (Biology).
-
Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
-
Greaves MF, Titley I, Colman SM, et al. CD34 cluster workshop report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:840-846.
-
Langenmayer I, Weaver C, Buckner CD, et al. Engraftment of patients with lymphoid malignancies transplanted with autologous bone marrow, peripheral blood stem cells or both. Bone Marrow Transplant. 1995; 15:241-246. (Biology).
-
Lowdell MW, Bainbridge DR. External quality assurance for CD34 cell enumeration–results of a preliminary national trial. Royal Microscopical Society Clinical Flow Cytometry Group QA Schemes. Bone Marrow Transplant. 1996; 17:849-853. (Biology).
-
Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard—Sixth Edition. H3-A6. 2007. (Biology).
-
Shpall EJ, Jones RB, Bearman SI, et al. Transplantation of enriched CD34-positive autologous marrow into breast cancer patients following high-dose chemotherapy: influence of CD34-positive peripheral-blood progenitors and growth factors on engraftment. J Clin Oncol. 1994; 12:28-36. (Biology).
-
Zander AR, Lyding J, Bielack S. Transplantation with blood stem cells. Blood Cells. 1991; 17:301-309. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For In Vitro Diagnostics Use.
