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FITC Mouse Anti-Rat CD32 D34-485 RUO

FITC Mouse Anti-Rat CD32 

Clone  D34-485   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor
  • Concentration 0.5 mg/ml
  • Isotype Mouse BALB/c IgG1, κ
  • Reactivity Rat (QC Testing) 
  • Application Flow cytometry (Routinely Tested) 
  • Immunogen Recombinant Rat CD32 Protein
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Detalhes do produto Recommended Assay Referências

Description

The D34-485 monoclonal antibody specifically recognizes CD32, the FcγII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 antibody blocks binding of aggregated immunoglobulins to the FcγII receptors in vitro.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

FORMATO
  • Format FITC
  • Excitation Source Blue 488 nm
  • Excitation Max 494 nm
  • Emission Max 520 nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

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Suggested Companion Products

FITC Mouse IgG1, κ Isotype Control RUO
0.25 mg Cat No: 550616

PE Mouse Anti-Rat IgM G53-238 RUO
0.2 mg Cat No: 553888

RECURSOS E FERRAMENTAS
 Spectrum Viewer  Panel Designer  Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

550272 Rev. 4
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